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SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

Cat. No. GTX136058-pro

Application

 Binding Assay, WB

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with E484K and N501Y mutations and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro WB Image

SARS-CoV-2 Spike RBD (E484K, N501Y), His tag protein (GTX136058-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Image

1 μg of GTX136058-pro SARS-CoV-2 Spike RBD (E484K, N501Y), His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active) (GTX136058-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

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REVIEW

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Package List Price ($)
$ 499