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SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

Cat No. GTX136058-pro

Application

Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS buffer. Reconstitute with distilled water.

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution and aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with E484K and N501Y mutations and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Image

SARS-CoV-2 Spike RBD (E484K, N501Y), His tag protein (GTX136058-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Image

1 μg of GTX136058-pro SARS-CoV-2 Spike RBD (E484K, N501Y), His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 with E484K, His tag (active). GTX136058-pro

GTX136058-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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