GeneTex
  • Country / Location Selection

United States (US)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

Cat No. GTX136022-pro

Application

ELISA, Functional Assay, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pair for sandwich ELISA:
Capture: GTX635807, Detection: GTX635792-01 / GTX635793-01

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water. Lyophilized from PBS buffer.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution and aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with K417N, E484K, and N501Y mutations and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Beta variant , B.1.351 , 501Y.v2 , SARS-CoV-2 beta , SARS2 beta

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Image

SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (GTX136022-pro, 1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Image

1μg of GTX136022-pro SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.43 μM

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 1.30 μM

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active) (GTX136022-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active) (GTX136022-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 499