PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.
Concentration
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with L452R and E484Q mutations and His tag at the C-terminus
Expression System
HEK293 cells
Purity
>95%
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Kappa variant Spike , B.1.617.1 Spike , SARS-CoV-2 Kappa variant Spike , COVID-19 Kappa variant Spike
Research Area
DATA IMAGES
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GTX136299-pro WB Image
SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) protein (GTX136299-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.
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GTX136299-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX136299-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX136299-pro ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.
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GTX136299-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX136299-pro Image
1 μg of GTX136299-pro SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
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GTX136299-pro Binding Assay Image
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (13.72-10000 ng/mL). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
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GTX136299-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX136299-pro Binding Assay Image
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
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REFERENCE
There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.1 / Kappa variant, His tag (active) (GTX136299-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.1 / Kappa variant, His tag (active) (GTX136299-pro). Be the first to share your experience with this product.
