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SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro

Cat. No. GTX136332-pro

Reference 1

Application

 Binding Assay, WB, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with L452R and T478K mutations and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Delta variant Spike , B.1.617.2 Spike , SARS-CoV-2 Delta variant Spike , COVID-19 Delta variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro

GTX136332-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) (2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro

GTX136332-pro Binding Assay Image

Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro

GTX136332-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag protein (GTX136332-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro

GTX136332-pro Image

1 μg of GTX136332-pro SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active). GTX136332-pro

GTX136332-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) using the antibodies as follows.
Capture : SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Contact us for custom HRP-conjugated antibody.

REFERENCE

Application Reference

Chiu CH et al. Microbiol Spectr 2022; 10 (5) : e0060922 Boosting with Multiple Doses of mRNA Vaccine after Priming with Two Doses of Protein Subunit Vaccine MVC-COV1901 Elicited Robust Humoral and Cellular Immune Responses against Emerging SARS-CoV-2 Variants.
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REVIEW

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Package List Price ($)
$ 499