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SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

Cat No. GTX136716-pro

Application

Binding Assay, ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX635807, Detection : GTX635792-01.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y and Y505H mutations, and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Omicron variant Spike , BA.1 Spike , SARS-CoV-2 Omicron variant Spike , COVID-19 Omicron variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins derived from different strains of SARS-CoV-2 (ie., wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant, B.1.617.1 Kappa variant; B.1.1.529 Omicron variant) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro Image

GTX136716-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag protein (GTX136716-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro Binding Assay Image

Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag. GTX136716-pro

GTX136716-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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Package List Price ($)
$ 499