SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag

Cat. No. GTX136716-pro

Reference 4

Application	WB, ELISA, Sandwich ELISA
Species	SARS Coronavirus 2
Package	100 μg ($499)

See all SARS-CoV-2 (COVID-19) Spike products

Datasheet
Reference

View product citations for antibody GTX136716-pro on CiteAb

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX635807, Detection : GTX635792-01.

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Trehalose, 5% Mannitol, 0.01% Tween-80

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y and Y505H mutations, and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Omicron variant Spike , BA.1 Spike , SARS-CoV-2 Omicron variant Spike , COVID-19 Omicron variant Spike

Research Area

DATA IMAGES

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins derived from different strains of SARS-CoV-2 (ie., wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant, B.1.617.1 Kappa variant; B.1.1.529 Omicron variant) using SARS-CoV-2 (COVID-19) Spike RBD Protein Sandwich ELISA Kit (GTX536267).

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX136716-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

GTX136716-pro Image

GTX136716-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

GTX136716-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136716-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136716-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag protein (GTX136716-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

GTX136716-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136716-pro ELISA Image

REFERENCE

Application Reference

Ho CL et al. Front Immunol 2024; 15 : 1340645 Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens.

TW Liu et al. J Ethnopharmacol 2024; 320 : 117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein

CL Ho et al. Front Immunol 2023; Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens

Application : ELISA

Tsilioni I et al. Int J Mol Sci 2023; 24 (11) Recombinant SARS-CoV-2 Spike Protein Stimulates Secretion of Chymase, Tryptase, and IL-1β from Human Mast Cells, Augmented by IL-33.

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REVIEW

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Application Reference

Ho CL et al. Front Immunol 2024; 15:1340645 Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens.

TW Liu et al. J Ethnopharmacol 2024; 320:117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein

CL Ho et al. Front Immunol 2023; Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens

Application : ELISA

Tsilioni I et al. Int J Mol Sci 2023; 24 (11) Recombinant SARS-CoV-2 Spike Protein Stimulates Secretion of Chymase, Tryptase, and IL-1β from Human Mast Cells, Augmented by IL-33.

Package	List Price ($)
100 μg	$ 499
Qty

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.1 variant, His tag

Cat. No. GTX136716-pro

Application

Species

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Region/Sequence

Expression System

Purity

Conjugation

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX136716-pro ELISA Image

GTX136716-pro ELISA Image

GTX136716-pro ELISA Image

GTX136716-pro ELISA Image

GTX136716-pro Image

GTX136716-pro ELISA Image

GTX136716-pro ELISA Image

GTX136716-pro WB Image

GTX136716-pro ELISA Image

GTX136716-pro ELISA Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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