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SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro

Cat. No. GTX137422-pro

Application

Binding Assay, WB, ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a.) with G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H and a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro

GTX137422-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag (GTX137422-pro) (148.15-2.31 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike RBD antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro

GTX137422-pro Image

GTX137422-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro

GTX137422-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag (1 μg, GTX137422-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro

GTX137422-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag. GTX137422-pro

GTX137422-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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Package List Price ($)
$ 499