SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag

Cat. No. GTX137422-pro

Application	Binding Assay, WB, ELISA, Sandwich ELISA
Species	SARS Coronavirus 2
Package	100 μg ($499)

See all SARS-CoV-2 (COVID-19) Spike products

Datasheet

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a.) with G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H and a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX137422-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag (GTX137422-pro) (148.15-2.31 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike RBD antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX137422-pro Image

GTX137422-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

GTX137422-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag (1 μg, GTX137422-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX137422-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX137422-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 499

Qty

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag

Cat. No. GTX137422-pro

Application

Species

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Region/Sequence

Expression System

Purity

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX137422-pro ELISA Image

GTX137422-pro Image

GTX137422-pro WB Image

GTX137422-pro ELISA Image

GTX137422-pro Binding Assay Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.