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SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

Cat No. GTX136905-pro

Application

 WB, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX635807, Detection : GTX635792-01.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 50% glycerol in distilled water to 0.1-1mg/ml. Lyophilized from buffer

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H mutations, and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Omicron variant Spike , BA.2 Spike , SARS-CoV-2 Omicron variant Spike , COVID-19 Omicron variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (GTX136905-pro) (143.73-2.25 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro Image

GTX136905-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (1 μg, GTX136905-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (GTX136905-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

REFERENCE

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REVIEW

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Package List Price ($)
$ 499