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SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro

Cat. No. GTX137879-pro

Reference 1

Application

 Binding Assay, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Capture : GTX635807, Detection : GTX637591 / GTX637592. Please notice that GTX637591 / GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.8 mg/ml. Lyophilized from PBS, 2% Trehalose, 5% Mannitol, 0.01% Tween-80.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a.) with G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H and a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro

GTX137879-pro Image

GTX137879-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro

GTX137879-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Please notice that GTX637591 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro

GTX137879-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (-10000, 3333, 1111, 370, 123, 41, 13 ng/ml ). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BQ.1 variant, His tag. GTX137879-pro

GTX137879-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (1 μg/mL)
Please notice that GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

REFERENCE

Application Reference

CL Ho et al. Front Immunol 2023; Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens
Application : ELISA
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Package List Price ($)
$ 499