Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB.1.5 variant, His tag (GTX138287-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Please notice that GTX637591 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Omicron / XBB variant; Omicron / XBB.1.5 variant) (143.88-2.25 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX138287-pro SARS-CoV-2 spike (RBD) XBB.1.5 variant, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.