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SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro

Cat. No. GTX138115-pro

Reference 1

Application

 ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA: Capture : GTX635807, Detection : GTX635793-01 / GTX637591

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Trehalose, 5% Mannitol, 0.01% Tween-80

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a.) with R346T, G339H, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H and a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro

GTX138115-pro Image

GTX138115-pro SARS-CoV-2 (COVID-19) Spike (RBD) Protein, XBB variant, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro

GTX138115-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Please notice that GTX637591 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro

GTX138115-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / XBB variant, His tag. GTX138115-pro

GTX138115-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Omicron / XBB variant; Omicron / XBB.1.5 variant) (143.88-2.25 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

REFERENCE

Application Reference

CL Ho et al. Front Immunol 2023; Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens
Application : ELISA
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Package List Price ($)
$ 499