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SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449]

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in Western Blot (WB). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

Cat. No. GTX636042

Host

Mouse

Clonality

Monoclonal

Clone Name

GT5449

Isotype

IgG2a

Application

WB, ELISA, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654.
Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) Spike RBD recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2909940

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in Western Blot (WB). GTX636042

GTX636042 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] used in ELISA (ELISA). GTX636042

GTX636042 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations