SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449]

Cat. No. GTX636042

Host	Mouse
Clonality	Monoclonal
Clone Name	GT5449
Isotype	IgG2a
Application	WB, ELISA, Sandwich ELISA
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

Datasheet

View product citations for antibody GTX636042 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654. Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) Spike RBD recombinant protein. (strain:Wuhan-hu-1)

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2909940

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL)

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX636042 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX636042 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

GTX636042 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449]

Cat. No. GTX636042

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 WB Image

GTX636042 ELISA Image

GTX636042 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.