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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

Cat No. GTX635791

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1002

Isotype

IgG

Application

ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604 / GTX635792 / GTX635807, Detection: GTX635791. or Capture : GTX635791, Detection: GTX635807 / GTX635792-01.Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792 or GTX635807. GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635791. Please contact us for conjugation service.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-coV-2 RBD recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791

GTX635791 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (13.72-10000 ng/mL). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) using the antibodies as follows.
Capture : SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791

GTX635791 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD proteins of WT and viral variants (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791

GTX635791 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK (Kent) variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791

GTX635791 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 spike (trimer) protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635791. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635791. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL).

Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791

GTX635791 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Spike S1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R), His Tag (delta variant) (52.15-0.81 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635791

GTX635791 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, AY.1 / Delta plus variant, His tag (delta plus variant) (GTX136333-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] used in ELISA (ELISA). GTX635791

GTX635791 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

REFERENCE

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REVIEW

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