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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792

Cat No. GTX635792

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1003

Isotype

IgG

Application

ICC/IF, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Neutralizing/Inhibition
This antibody inhibits infection of mammalian cells by live SARS-CoV-2.

Sandwich ELISA
Capture : GTX632604 / GTX635807, Detection : GTX635792

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV spike protein based on customer’s feedback.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 Spike RBD. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888574

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792

GTX635792 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (20-4.8*10-6 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 7.49 pM

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792

GTX635792 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635792

GTX635792 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) diluted at 1:700.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792

GTX635792 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635792 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792

GTX635792 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635792 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792

GTX635792 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792

GTX635792 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792

GTX635792 Neutralizing/Inhibition Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) effectively neutralizes SARS-CoV-2 pseudovirus entry into human cells. Control or tested antibodies were mixed with GFP-expressing SARS-CoV-2 (wild-type) pseudovirus. Each mixture was then added to 293T cells overexpressing human ACE2. After 48 hours, the infections were observed by detecting GFP using fluorescence microscopy.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792

GTX635792 Neutralizing/Inhibition Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) effectively neutralizes SARS-CoV-2 pseudovirus entry into human cells. Control or tested antibodies were mixed with GFP-expressing SARS-CoV-2 (Furin modified) pseudovirus. Each mixture was then added to 293T cells overexpressing human ACE2. After 48 hours, the infections were observed by detecting GFP using fluorescence microscopy.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792). Be the first to share your publications with this product.

REVIEW

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] Cat No. GTX635792

Rating ( Average 5 based on 2 users reviews)
Application
Neutralizing/Blocking/Inhibition(Neutralizing/Inhibition) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 27-Jan-2021
Score :
Application Tested : Neutralizing/Inhibition
Sample Species : SARS-CoV-2
Sample : ACE2-293T cells
Date : Anonymous submitted on 27-Jan-2021
Score :
Application Tested : Neutralizing/Inhibition
Sample Species : SARS-CoV-2
Sample : ACE2-293T cells