SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003]

Name: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003]
Brand: GeneTex
SKU: GTX635792
Price: 369.00 USD
Rating: 5 (2 reviews)

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in ELISA (ELISA). GTX635792

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635792

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635792

Cat No. GTX635792

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1003
Isotype	IgG
Application	ICC/IF, ELISA, Neutralizing/Inhibition, Sandwich ELISA
Reactivity	SARS Coronavirus 2
Package	100 μl ($369), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	1:100-1:1000
ELISA	Assay dependent
Neutralizing/Inhibition	Assay dependent
Sandwich ELISA	Assay dependent
Note : Neutralizing/Inhibition This antibody inhibits infection of mammalian cells by live SARS-CoV-2. Sandwich ELISA Capture : GTX632604 / GTX635807, Detection : GTX635792

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV spike protein based on customer’s feedback.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 Spike RBD. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888574

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

GTX635792 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (20-4.8*10^-6 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 7.49 pM

GTX635792 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635792 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) diluted at 1:700.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635792 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635792 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635792 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635792 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635792 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

GTX635792 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX635792 Neutralizing/Inhibition Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) effectively neutralizes SARS-CoV-2 pseudovirus entry into human cells. Control or tested antibodies were mixed with GFP-expressing SARS-CoV-2 (wild-type) pseudovirus. Each mixture was then added to 293T cells overexpressing human ACE2. After 48 hours, the infections were observed by detecting GFP using fluorescence microscopy.

GTX635792 Neutralizing/Inhibition Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) effectively neutralizes SARS-CoV-2 pseudovirus entry into human cells. Control or tested antibodies were mixed with GFP-expressing SARS-CoV-2 (Furin modified) pseudovirus. Each mixture was then added to 293T cells overexpressing human ACE2. After 48 hours, the infections were observed by detecting GFP using fluorescence microscopy.

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REVIEW