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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

Cat No. GTX635793

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1004

Isotype

IgG

Application

ICC/IF, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Neutralizing/Inhibition
This antibody inhibits infection of mammalian cells by live SARS-CoV-2.

Sandwich ELISA
Capture : GTX632604 / GTX635807, Detection : GTX635793

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV spike protein based on customer’s feedback. This antibody is able to detect SARS-CoV-2 Spike RBD (N501Y mutant) protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 Spike RBD. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888576

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635793

GTX635793 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) diluted at 1:600.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (160-1.91*10-5 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 25.13 pM

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635793

GTX635793 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000)

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] used in ELISA (ELISA). GTX635793

GTX635793 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 (COVID-19) Spike (GTX535664), MERS-CoV Spike (GTX535667), or SARS-CoV Spike (GTX535668) transfected 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793). Be the first to share your publications with this product.

REVIEW

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