SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014]

Cat. No. GTX635807

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1014
Isotype	IgG
Application	ICC/IF, ELISA, Sandwich ELISA
Reactivity	SARS Coronavirus 2
Package	100 μl ($419), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

Datasheet
Reference

View product citations for antibody GTX635807 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	1:100-1:2500
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635807, Detection : GTX635792-01 / GTX635793-01 / GTX636042 / GTX637591 / GTX637592 /GTX635791-01 or Capture : GTX636042 / GTX635791 / GTX635792 / GTX635793, Detection : GTX635807. Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792 / GTX635793. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variants (BA.1, BA.2, and XBB.1.5).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 Spike RBD (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888580

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.12.1 variant, His tag (GTX137114-pro) (28.62-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.75 variant, His tag (GTX137422-pro) (148.15-2.31 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike RBD antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (GTX136905-pro) (143.73-2.25 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag (GTX137533-pro) using ELISA pairs below.
Pair 1:
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL)
Pair 2:
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL)
Pair 3:
Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL)
Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792, GTX635793 and GTX637592. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.75 variant, His tag (GTX137533-pro) using ELISA pairs below.
Pair 1:
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Pair 2:
Capture: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Pair 3:
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (1 μg/mL)
Please notice that GTX637591 and GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807 and GTX637592. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2.12.1 variant, His tag (GTX137249-pro) (143.68-2.24 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike RBD antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Please notice that GTX637591 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Omicron / XBB variant; Omicron / XBB.1.5 variant) (143.88-2.25 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (GTX137880-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/ BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (160-1.91*10^-5 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 28.77 pM

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (HexaPro) ECD, His tag (GTX135972-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 (COVID-19) Spike (GTX535664), MERS-CoV Spike (GTX535667), or SARS-CoV Spike (GTX535668) transfected 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635792 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) diluted at 1:2500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635807 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 (COVID-19) Spike (GTX535664), MERS-CoV Spike (GTX535667), or SARS-CoV Spike (GTX535668) transfected 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136022-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10^-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.43 μM

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag (GTX136716-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL).
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 μg/mL.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10^-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.24 μM

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, mouse Fc tag (GTX136024-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag (active) (GTX02575-pro), and SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) using the antibodies as follows.
Capture : SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Spike S1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R), His Tag (delta variant) (52.15-0.81 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635791. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (1 μg/mL)
Please notice that GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (GTX637591) (1 μg/mL)
Please notice that GTX637591 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; C.37 lambda variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (HRP) (GTX635791-01) (1 μg/mL)

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, P.1 / Gamma variant, His tag (active) (GTX136043-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (HRP) (GTX635791-01) (1 μg/mL)

GTX635807 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BF.7 variant, His tag (GTX137878-pro) (148.15-2.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635792 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (GTX137880-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (1 μg/mL)
Please notice that GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (1 μg/mL)
Please notice that GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX635807 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL)
Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635793. Please contact us for custom HRP-conjugated antibody.

REFERENCE

Application Reference

Ambro?ek-Latecka M et al. Cell Death Discov 2024; 10 (1) : 191 SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1α in pulmonary epithelial and endothelial cells.

Application : ICC/IF

Submit a Reference

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807). Be the first to share your experience with this product.

Submit a Review

Application Reference

Ambro?ek-Latecka M et al. Cell Death Discov 2024; 10 (1):191 SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1α in pulmonary epithelial and endothelial cells.

Application : ICC/IF

Package	List Price ($)
100 μl	$ 419
25 μl	$ 169
Qty Custom Conjugations

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014]

Cat. No. GTX635807

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