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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in ELISA (ELISA). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in ELISA (ELISA). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

Cat. No. GTX635692

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL257

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:500-1:3000
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes.

Not tested in other applications.

Positive Control

SARS-CoV-2 spike (Full length)-transfected 293T

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692

GTX635692 IHC-P Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded non-infected (left) and infected (right) human placenta.
SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (1 μg, GTX137037-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Various SARS-CoV-2 (COVID-19) Spike RBD proteins were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692

GTX635692 IHC-P Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded non-infected (left) and infected (right) human lung.
SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635692

GTX635692 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in ELISA (ELISA). GTX635692

GTX635692 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) protein (GTX01546-pro) (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 Spike S1 (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G), South Africa variant, His tag (0.25 μg, GTX136095-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (1 μg, GTX136905-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (0.5 μg, GTX135972-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in ELISA (ELISA). GTX635692

GTX635692 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (159.36-2.49 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Various SARS-CoV-2 (COVID-19) Spike RBD proteins were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635692

GTX635692 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) and SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (GTX136085-pro) (0.5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136780-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.529 / Omicron variant, His tag protein (GTX136716-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692

GTX635692 IHC-P Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded infected lung tissue derived from the AdV-hACE2-transduced BALB/c mice model.
SARS-CoV-2 (COVID-19) Spike RBD stained by IgG control (left) and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000 (right).
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 Spike S1 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), Brazil variant, His tag (0.25 μg, GTX136094-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635692

GTX635692 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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Package List Price ($)
$ 399
$ 169
Custom Conjugations