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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in ELISA (ELISA). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in  (). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635692

Cat No. GTX635692

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL257

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:500-1:3000
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes.

Not tested in other applications.

Positive Control

293T , SARS-CoV-2 spike (Full length)-transfected 293T , SARS-CoV-2 (2019-nCoV) Spike

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888564

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692

GTX635692 IHC-P Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded non-infected (left) and infected (right) human placenta.
SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692

GTX635692 IHC-P Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded non-infected (left) and infected (right) human lung.
SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in ELISA (ELISA). GTX635692

GTX635692 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) protein (GTX01546-pro) (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635692

GTX635692 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC (Paraffin sections) (IHC-P). GTX635692

GTX635692 IHC-P Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunohistochemical analysis.
Sample: Paraffin-embedded infected lung tissue derived from the AdV-hACE2-transduced BALB/c mice model.
SARS-CoV-2 (COVID-19) Spike RBD stained by IgG control (left) and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000 (right).
Antigen Retrieval: Citrate buffer, pH 6.0, 20 min

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in  (). GTX635692

GTX635692 Image

SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) and SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (GTX136085-pro) (0.5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 Spike S1 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), Brazil variant, His tag (0.25 μg, GTX136094-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in Western Blot (WB). GTX635692

GTX635692 WB Image

SARS-CoV-2 Spike S1 (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G), South Africa variant, His tag (0.25 μg, GTX136095-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635692

GTX635692 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] detects SARS-CoV-2 (COVID-19) Spike RBD protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692). Be the first to share your publications with this product.

REVIEW

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SDS
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