SARS-CoV-2 (COVID-19) Spike RBD antibody

Cat No. GTX135709

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS, ELISA, Neutralizing/Inhibition, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($359), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
FACS	Assay dependent
ELISA	Assay dependent
Neutralizing/Inhibition	Assay dependent
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 (COVID-19) Spike RBD. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887506

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

GTX135709 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX135709 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike RBD antibody detects SARS-CoV-2 (COVID-19) Spike RBD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX135709 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX135709 Neutralizing/Inhibition Image

Inhibition ELISA analysis. Immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) (GTX01548-pro) (coated at 2 μg/mL) was bound to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL) with the presence of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL). The bound ACE2 protein was detected by the Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

GTX135709 FACS Image

SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) detects SARS-CoV-2 (COVID-19) Spike RBD protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX135709 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX135709 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 359

25 μl

$ 169

Qty

SARS-CoV-2 (COVID-19) Spike RBD antibody

Cat No. GTX135709

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX135709 WB Image

GTX135709 IHC-P (cell pellet) Image

GTX135709 WB Image

GTX135709 Neutralizing/Inhibition Image

GTX135709 FACS Image

GTX135709 ICC/IF Image

GTX135709 ELISA Image

REFERENCE

REVIEW

SARS-CoV-2 (COVID-19) Nucleocapsid Protein Sandwich ELISA Kit

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511]

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

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