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SARS-CoV-2 (COVID-19) Spike RBD antibody

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135709
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135709
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135709
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX135709
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Flow cytometry (FACS). GTX135709
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135709
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in ELISA (ELISA). GTX135709

Cat No. GTX135709

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, ELISA, Neutralizing/Inhibition, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
FACS Assay dependent
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 (COVID-19) Spike RBD. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887506

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135709

GTX135709 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135709

GTX135709 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike RBD antibody detects SARS-CoV-2 (COVID-19) Spike RBD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135709

GTX135709 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX135709

GTX135709 Neutralizing/Inhibition Image

Inhibition ELISA analysis. Immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) (GTX01548-pro) (coated at 2 μg/mL) was bound to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL) with the presence of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL). The bound ACE2 protein was detected by the Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Flow cytometry (FACS). GTX135709

GTX135709 FACS Image

SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) detects SARS-CoV-2 (COVID-19) Spike RBD protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135709

GTX135709 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike RBD antibody detects SARS-CoV-2 (COVID-19) Spike RBD protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike RBD stained by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in ELISA (ELISA). GTX135709

GTX135709 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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