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SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

Cat No. GTX01546-pro

Application

Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 (COVID-19) Spike RBD protein at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 8-80 ng/mL.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 0.22 μm-filtered PBS (pH 7.4). Reconstitute with distilled water to a concentration of 0.1-0.5 mg/mL.

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Arg319-Phe541 of SARS-CoV-2 (COVID-19) Spike RBD protein (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.

Expression System

HEK293 Cells

Purity

> 95% by SDS-PAGE

Endotoxin

< 0.1 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Database

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Image

SDS-PAGE of GTX01546-pro SARS-CoV-2 (COVID-19) Spike RBD protein (active).

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro). Be the first to share your experience with this product.
SDS
PBS.pdf