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SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

Cat. No. GTX136085-pro

Application

 Binding Assay, WB

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-685 a.a) with mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H) and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Alpha variant Spike , B.1.1.7 Spike , SARS-CoV-2 Alpha variant Spike , COVID-19 Alpha variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Image

1 μg of GTX136085-pro SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike S1 protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active) (GTX136085-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro WB Image

SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) protein (GTX136085-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

REFERENCE

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REVIEW

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Package List Price ($)
$ 499