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SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro
SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

Cat No. GTX136085-pro

Application

Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-685 a.a) with mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H) and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 alpha , SARS2 alpha , B.1.1.7 , 501Y.v1

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Image

1 μg of GTX136085-pro SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Image

SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) protein (GTX136085-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136085-pro

GTX136085-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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