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SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263]

Comparison Orthogonal
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Western Blot (WB). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Western Blot (WB). GTX635708
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Western Blot (WB). GTX635708

Cat. No. GTX635708

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT263

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX635708, Detection: GTX635672
Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) Spike (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888568

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708

GTX635708 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708

GTX635708 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635708

GTX635708 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708

GTX635708 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Western Blot (WB). GTX635708

GTX635708 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in ELISA (ELISA). GTX635708

GTX635708 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635708

GTX635708 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Western Blot (WB). GTX635708

GTX635708 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] used in Western Blot (WB). GTX635708

GTX635708 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shapira T et al. Mol Biomed 2022; 3 (1) : 43 Inhibition of glycogen synthase kinase-3-beta (GSK3β) blocks nucleocapsid phosphorylation and SARS-CoV-2 replication.
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations