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SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

Cat No. GTX635656

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604, Detection: GTX635656

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminal region of SARS-CoV-2 (COVID-19) spike. (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888549

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635656) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635656

GTX635656 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL1] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL1] (GTX635656) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635656 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.117 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656

GTX635656 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656

GTX635656 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

REVIEW

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SDS
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