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SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

Cat. No. GTX635672

Reference 3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL263

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX635708 / GTX632604, Detection : GTX635672
Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888551

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635672

GTX635672 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.365 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672

GTX635672 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672

GTX635672 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635672

GTX635672 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672

GTX635672 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL).

REFERENCE

Application Reference

Tien CF et al. iScience 2022; 25 (8) : 104709 Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking.
Application : ICC/IF
Welch JL et al. J Infect Dis 2021; (Epub) Human T cells express Angiotensin Converting Enzyme 2 at levels sufficient to interact with the SARS-CoV-2 Spike protein.
Leonid Yurkovetskiy et al. Cell 2020; S0092-8674 (20) : 31229-0 Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations