SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

Cat No. GTX635672

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL263
Isotype	IgG
Application	WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($369), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : Sandwich ELISA Capture : GTX635708/GTX632604, Detection: GTX635672

Not tested in other applications.

Specificity/Sensitivity

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1). (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

GTX635672 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635672 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635672 ELISA Image

GTX635672 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635672 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635672 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635672 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.365 nM

GTX635672 WB Image

REFERENCE

Application Reference

Leonid Yurkovetskiy et al. Cell 2020; S0092-8674 (20) : 31229-0 Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.

Application : WB

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REVIEW

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Application Reference

Leonid Yurkovetskiy et al. Cell 2020; S0092-8674 (20):31229-0 Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.

Application : WB

SDS
PBS.pdf

Package	List Price ($)
100 μl	$ 369
25 μl	$ 169
Qty

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

Cat No. GTX635672

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Specificity/Sensitivity

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX635672 ELISA Image

GTX635672 ELISA Image

GTX635672 ELISA Image

GTX635672 ICC/IF Image

GTX635672 IHC-P (cell pellet) Image

GTX635672 ELISA Image

GTX635672 WB Image

GTX635672 ELISA Image

GTX635672 WB Image

REFERENCE

Application Reference

REVIEW

SARS-CoV-2 (COVID-19) Nucleocapsid Protein Sandwich ELISA Kit

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511]

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