SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

Cat. No. GTX635672
Cat. No.  GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635672
GTX635672 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672
GTX635672 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672
GTX635672 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.365 nM

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL).

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635672
GTX635672 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
GTX635672 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in Western Blot (WB). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL263
  • Isotype
    IgG
  • Applications
    WB ICC/IF FACS ELISA Sandwich ELISA IHC-P (cell pellet)
  • Reactivity
    SARS Coronavirus 2
See all SARS-CoV-2 (COVID-19) Spike products