SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]

Name: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]
Brand: GeneTex
SKU: GTX635654
Price: 399.00 USD

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654

Cat. No. GTX635654

Reference 19

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL6
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:500
FACS	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes , background Eraser (BGE) blocking step, and DAB Enhancer. Sandwich ELISA Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654. Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888548

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.251 nM

GTX635654 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635654) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635654 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635654 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635654 FACS Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:2000.

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

GTX635654 WB Image

REFERENCE

Application Reference

K Miura et al. J Virol 2023; Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells

Application : WB

C Di Primio et al. PNAS Nexus 2023; 2 (9) : pgad282 Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons

Application : WB

Liu JF et al. EBioMedicine 2023; 90 : 104518 Proteomic and phosphoproteomic characteristics of the cortex, hippocampus, thalamus, lung, and kidney in COVID-19-infected female K18-hACE2 mice.

Piranej S et al. bioRxiv 2023; Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.

Nasser H et al. STAR Protoc 2022; 3 (4) : 101773 Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay.

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REVIEW

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Application Reference

K Miura et al. J Virol 2023; Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells

Application : WB

C Di Primio et al. PNAS Nexus 2023; 2 (9):pgad282 Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons

Application : WB

Liu JF et al. EBioMedicine 2023; 90:104518 Proteomic and phosphoproteomic characteristics of the cortex, hippocampus, thalamus, lung, and kidney in COVID-19-infected female K18-hACE2 mice.

Piranej S et al. bioRxiv 2023; Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.

Reyes S et al. Sens Diagn 2022; 1 (6):1198-1208 Metal organic framework encapsulated tamavidin-Gluc reporter: application in COVID-19 spike antigen bioluminescent immunoassay.

Application : Sandwich ELISA

Padula L et al. Vaccine X 2022; 12:100202 Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses.

Application : ICC/IF