SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]

Name: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]
Brand: GeneTex
SKU: GTX635654
Price: 399.00 USD

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654

Cat. No. GTX635654

Reference 17

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL6
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:500
FACS	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes , background Eraser (BGE) blocking step, and DAB Enhancer. Sandwich ELISA Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654. Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888548

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.251 nM

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX635654 FACS Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX635654 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635654) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635654 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635654 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:2000.

GTX635654 WB Image

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

GTX635654 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

REFERENCE

Application Reference

Liu JF et al. EBioMedicine 2023; 90 : 104518 Proteomic and phosphoproteomic characteristics of the cortex, hippocampus, thalamus, lung, and kidney in COVID-19-infected female K18-hACE2 mice.

Piranej S et al. bioRxiv 2023; Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.

Padula L et al. Vaccine X 2022; 12 : 100202 Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses.

Application : ICC/IF

Chuang HC et al. EMBO Mol Med 2022; 14 (9) : e15904 SARS-CoV-2 spike protein enhances MAP4K3/GLK-induced ACE2 stability in COVID-19.

Nasser H et al. STAR Protoc 2022; 3 (4) : 101773 Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay.

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REVIEW

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Application Reference

Liu JF et al. EBioMedicine 2023; 90:104518 Proteomic and phosphoproteomic characteristics of the cortex, hippocampus, thalamus, lung, and kidney in COVID-19-infected female K18-hACE2 mice.

Piranej S et al. bioRxiv 2023; Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.

Reyes S et al. Sens Diagn 2022; 1 (6):1198-1208 Metal organic framework encapsulated tamavidin-Gluc reporter: application in COVID-19 spike antigen bioluminescent immunoassay.

Padula L et al. Vaccine X 2022; 12:100202 Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses.

Application : ICC/IF

Chuang HC et al. EMBO Mol Med 2022; 14 (9):e15904 SARS-CoV-2 spike protein enhances MAP4K3/GLK-induced ACE2 stability in COVID-19.

Nasser H et al. STAR Protoc 2022; 3 (4):101773 Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay.

Yuchao Chen et al. Sci Adv 2022; 8 (21):eabn3481 Quantitative and ultrasensitive in situ immunoassay technology for SARS-CoV-2 detection in saliva.

Wei Deng et al. Signal Transduct Target Ther 2022; 7 (1):124 Sequential immunizations confer cross-protection against variants of SARS-CoV-2, including Omicron in Rhesus macaques.

Application : IHC-P