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SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

Cat No. GTX635654

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL6

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604, Detection: GTX635654

Not tested in other applications.

Specificity/Sensitivity

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1). (SARS-CoV-2 (strain Wuhan-Hu-1)) The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635654) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635654 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654

GTX635654 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654

GTX635654 FACS Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (2019-nCoV) Spike-transfected 293T (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.251 nMIndirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.251 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
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