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SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

Cat. No. GTX635654

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL6

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:500
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes , background Eraser (BGE) blocking step, and DAB Enhancer.

Sandwich ELISA
Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654.
Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888548

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.251 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654

GTX635654 FACS Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635654) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635654 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:2000.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654

GTX635654 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations