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SARS-CoV-2 (COVID-19) Spike S1 overexpression 293T whole cell lysate

SARS-CoV-2 (COVID-19) Spike S1 overexpression 293T whole cell lysate. GTX535663
SARS-CoV-2 (COVID-19) Spike S1 overexpression 293T whole cell lysate. GTX535663

Cat No. GTX535663

Application

WB

Species

SARS Coronavirus 2
Package
200 μg ($299)
PRODUCT

Summary

Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 spike S1 subunit (C-terminal His-tagged). The translated amino acid sequence is based on QHD43416.1 (a.a. 1-685). Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A, 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S1 overexpression 293T whole cell lysate. GTX535663

GTX535663 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 overexpression 293T whole cell lysate. GTX535663

GTX535663 WB Image

SARS-CoV-2 (COVID-19) spike S1 293T overexpression cell lysates (GTX535663) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Proclin.pdf