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SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active)

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

Cat No. GTX01554-pro

Application

ELISA, Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 μg/ml (100 μl/well ) can bind Recombinant nCoV-S1. The EC₅₀ of SARS-CoV-2 (COVID-19) Spike S1 protein is 0.25-0.45 μg/ml.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 0.22 μm filtered PBS (pH 7.4). Reconstitute with distilled water to a concentration of 0.1-0.5 mg/ml.

Storage

Store at -20ºC or below. After reconstitution, the protein solution is stable at -20 ºC for 1 month, at 4 ºC for up to 1 week. Avoid freeze-thaw cycles.

Region/Sequence

Val16-Arg685 of SARS-CoV-2 (COVID-19) Spike S1 protein (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.

Expression System

HEK293 Cells

Purity

> 90% by SDS-PAGE

Endotoxin

< 1.0 EU/μg (determined by the LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Database

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro ELISA Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX01554-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody (GTX135384) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro Image

SDS-PAGE of GTX01554-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active).

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro). Be the first to share your experience with this product.
SDS
PBS.pdf