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SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active)

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

Cat. No. GTX135817-pro

Reference 1

Application

 Binding Assay, WB, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($439)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Recommended antibody pair for sandwich ELISA: Capture: GTX635807, Detection: GTX635792-01 / GTX635793-01/ GTX635791.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-685 a.a) with His tag at the C-terminus

Expression System

HEK293 cells

Purity

>90%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro ELISA Image

Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro ELISA Image

Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (52.15-0.81 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike S1 protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.351 beta variant; P.1 gamma variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro WB Image

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro Image

1 μg of GTX135817-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro ELISA Image

Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL).

Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro Image

1 μg of GTX135817-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX135817-pro

GTX135817-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX135817-pro, 1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wei WC et al. Pharmacol Res 2022; 184 : 106424 Targeting spike protein-induced TLR/NET axis by COVID-19 therapeutic NRICM102 ameliorates pulmonary embolism and fibrosis.
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REVIEW

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Package List Price ($)
$ 439