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SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro
SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro
SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro
SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro

Cat No. GTX135684-pro

Application

ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pair for sandwich ELISA:
Capture: GTX632604, Detection: GTX635693 / GTX635910

PROPERTIES

Form

Liquid

Buffer

Lyophilized from PBS buffer. Reconstitute with distilled water.

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Region/Sequence

SARS-CoV-2 Spike of YP_009724390.1 (686-1213 a.a, extracellular domain of Spike S2) with the Fc region of mouse IgG1 at the C-terminus

Expression System

HEK293 cells

Purity

>90%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro

GTX135684-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro

GTX135684-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro

GTX135684-pro Image

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag. GTX135684-pro

GTX135684-pro Image

1μg of GTX135684-pro SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

REFERENCE

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REVIEW

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