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SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag

SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro
SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro
SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro
SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro
SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro

Cat No. GTX136023-pro

Application

ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pair for sandwich ELISA:
Capture: GTX632604, Detection: GTX635911 / GTX635910 / GTX635693

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (686-1,213 a.a) with T716I, S982A and D1118H mutations, 6 stabilizing mutations (HexaPro mutations, including F817P, A892P, A899P, A942P, K986P, and V987P), and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 alpha , SARS2 alpha , B.1.1.7 , 501Y.v1

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro

GTX136023-pro Image

SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane were blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910, left) diluted at 1:20000, SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (GTX635911, middle) diluted at 1:20000, and SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604, right) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro

GTX136023-pro Image

1μg of GTX136023-pro SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro

GTX136023-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1039] (GTX635911) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro

GTX136023-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S2 Protein, B.1.1.7 / Alpha variant, His tag. GTX136023-pro

GTX136023-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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