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SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

Cat. No. GTX635910

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1038

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604 / GTX636037 / GTX636038 , Detection : GTX635910

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the extracellular domain of SARS-CoV-2 (COVID-19) Spike S2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909921

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635910

GTX635910 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635910

GTX635910 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635910

GTX635910 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910

GTX635910 WB Image

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL).

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910

GTX635910 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910

GTX635910 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S2 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations