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SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635910
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

Cat No. GTX635910

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1038

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:100000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604 / GTX636037 / GTX636038 , Detection : GTX635910

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of SARS-CoV-2 (COVID-19) Spike S2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL).

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635910

GTX635910 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910

GTX635910 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910

GTX635910 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Western Blot (WB). GTX635910

GTX635910 WB Image

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635910

GTX635910 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] used in ELISA (ELISA). GTX635910

GTX635910 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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