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SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237]

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693

Cat. No. GTX635693

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL237

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604 / GTX636042 , Detection: GTX635693

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S2). (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888565

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.12.1 variant, His tag (GTX137114-pro) (28.62-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635693

GTX635693 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein (GTX135684-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635693

GTX635693 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S2 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693

GTX635693 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (GTX137880-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693

GTX635693 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
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Package List Price ($)
$ 399
$ 169
Custom Conjugations