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SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693
Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635693

Cat No. GTX635693

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL237

Isotype

IgG

Application

WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604 / GTX636042 , Detection: GTX635693

Not tested in other applications.

Positive Control

293T , SARS-CoV-2 (2019-nCoV) Spike-transfected 293T

Product Note

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S2). (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888565

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635693

GTX635693 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693

GTX635693 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein (GTX135684-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Western Blot (WB). GTX635693

GTX635693 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in ELISA (ELISA). GTX635693

GTX635693 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635693

GTX635693 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
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