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SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664
SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664
SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

Cat No. GTX535664

Application

WB, ELISA

Species

SARS Coronavirus 2
Package
200 μg ($299)
PRODUCT

Summary

Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 spike protein (full-length, no tag). The translated amino acid sequence is based on QHD43416.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A, 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

GTX535664 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

GTX535664 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

GTX535664 WB Image

SARS-CoV-2 (COVID-19) spike 293T overexpression cell lysates (GTX535664) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Proclin.pdf