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SARS-CoV-2 (COVID-19) nsp10 antibody

Anti-SARS-CoV-2 (COVID-19) nsp10 antibody used in Western Blot (WB). GTX135733
Anti-SARS-CoV-2 (COVID-19) nsp10 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135733
Anti-SARS-CoV-2 (COVID-19) nsp10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135733

Cat. No. GTX135733

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nsp10 recombinant protein

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2909872

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp10 antibody used in Western Blot (WB). GTX135733

GTX135733 WB Image

Non-transfected (!V) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp10 antibody (GTX135733) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX

Anti-SARS-CoV-2 (COVID-19) nsp10 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135733

GTX135733 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nsp10 antibody detects SARS-CoV-2 (COVID-19) nsp10 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) nsp10 transfected 293T cell FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nsp10 stained by SARS-CoV-2 (COVID-19) nsp10 antibody (GTX135733) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nsp10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135733

GTX135733 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp10 antibody detects SARS-CoV-2 (COVID-19) nsp10 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp10 stained by SARS-CoV-2 (COVID-19) nsp10 antibody (GTX135733) diluted at 1:2000.

REFERENCE

Application Reference

Akula SM et al. RNA Biol 2021; : 1-11 Cellular miR-150-5p may have a crucial role to play in the biology of SARS-CoV-2 infection by regulating nsp10 gene.
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REVIEW

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Package List Price ($)
$ 399
$ 169