SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444]

Cat. No. GTX636916

GTX636916 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

1 / 3

GTX636916 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] detects SARS-CoV-2 (COVID-19) nsp12 / RdRP protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp12 / RdRP stained by SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

2 / 3

GTX636916 IHC-P (cell pellet) Image

SARS-CoV-2 nsp12 detects SARS-CoV-2 nsp12 protein at cytoplasm by immunohistochemical analysis.
Sample: human SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 nsp12 stained by SARS-CoV-2 nsp12 (GTX636916) diluted at 1:255.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

3 / 3

This SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) is a rabbit monoclonal antibody validated for detection of SARS-CoV-2 (COVID-19) nsp12 / RdRP by WB, ICC/IF, and IHC-P (cell pellet). Reactivity has been shown in SARS Coronavirus 2.

Host

Rabbit
Clonality

Monoclonal
Clone Name

HL1444
Isotype

IgG
Applications

WB ICC/IF IHC-P (cell pellet)
Reactivity

SARS Coronavirus 2