SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444]

Name: SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444]
Brand: GeneTex
SKU: GTX636916
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] used in Western Blot (WB). GTX636916

Anti-SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636916

Anti-SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636916

Cat. No. GTX636916

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1444
Isotype	IgG
Application	WB, ICC/IF, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) nsp12 products

Datasheet

View product citations for antibody GTX636916 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp12 / RdRP. (Strain:Wuhan-hu-1)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636916 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636916 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] detects SARS-CoV-2 (COVID-19) nsp12 / RdRP protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp12 / RdRP stained by SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GTX636916 IHC-P (cell pellet) Image

SARS-CoV-2 nsp12 detects SARS-CoV-2 nsp12 protein at cytoplasm by immunohistochemical analysis.
Sample: human SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 nsp12 stained by SARS-CoV-2 nsp12 (GTX636916) diluted at 1:255.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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