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SARS-CoV-2 (COVID-19) nsp12 antibody

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135467
Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135467
Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135467
Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135467
Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135467

Cat No. GTX135467

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

SARS Coronavirus 2
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against N-terminal domain of nsp12 protein, and it does not react with RdRp enzyme domain.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) RdRp (nsp12) (Strain:Wuhan-Hu-1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887496

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV RdRp , SARS-CoV-2 RdRp , COVID-19 RdRp , 2019-nCoV RNA-directed RNA polymerase , SARS-CoV-2 RNA-directed RNA polymerase , COVID-19 RNA-directed RNA polymerase , 2019-nCoV nsp12 , SARS-CoV-2 nsp12 , COVID-19 nsp12

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135467

GTX135467 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135467) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135467

GTX135467 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp12 antibody detects SARS-CoV-2 (COVID-19) RdRp nsp12 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp12 stained by SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135467) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135467

GTX135467 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135467) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135467

GTX135467 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp12 antibody detects SARS-CoV-2 (COVID-19) nsp12 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp12 stained by SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135467) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135467

GTX135467 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135467) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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