APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
Not tested in other applications.
Product Note
This antibody was raised against N-terminal domain of nsp12 protein, and it does not react with RdRp enzyme domain.
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
SARS Coronavirus 2
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) nsp12 (Strain:Wuhan-Hu-1). The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2887497
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
2019-nCoV RdRp , SARS-CoV-2 RdRp , COVID-19 RdRp , 2019-nCoV RNA-directed RNA polymerase , SARS-CoV-2 RNA-directed RNA polymerase , COVID-19 RNA-directed RNA polymerase , 2019-nCoV nsp12 , SARS-CoV-2 nsp12 , COVID-19 nsp12
Research Area
DATA IMAGES
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GTX135469 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135469) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX135469 ICC/IF Image
SARS-CoV-2 (COVID-19) nsp12 antibody detects SARS-CoV-2 (COVID-19) nsp12 protein by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nsp12 stained by SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135469) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135469). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135469). Be the first to share your experience with this product.
