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SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] used in Western Blot (WB). GTX636917
Anti-SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636917
Anti-SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636917

Cat. No. GTX636917

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1445

Isotype

IgG

Application

WB, ICC/IF, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp15. (Strain:Wuhan-hu-1)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] used in Western Blot (WB). GTX636917

GTX636917 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (GTX636917) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636917

GTX636917 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] detects SARS-CoV-2 (COVID-19) nsp15 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp15 stained by SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (GTX636917) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636917

GTX636917 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] detects SARS-CoV-2 (COVID-19) nsp15 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) nsp15 transfected 293T.
Green: SARS-CoV-2 (COVID-19) nsp15 stained by SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (GTX636917) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] (GTX636917). Be the first to share your publications with this product.

REVIEW

SARS-CoV-2 (COVID-19) nsp15 antibody [HL1445] Cat. No. GTX636917

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-May-2022
Score :
Application Tested : WB
Sample Species : SARS-CoV-2
Sample : Vero E6 cells with/without SARS-CoV-2 (Omicron) infection, non-reducing
Primary Antibody : 1:1000, °C, Hr
Package List Price ($)
$ 399
$ 169
Custom Conjugations