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SARS-CoV-2 (COVID-19) nsp2 antibody

Anti-SARS-CoV-2 (COVID-19) nsp2 antibody used in Western Blot (WB). GTX135717
Anti-SARS-CoV-2 (COVID-19) nsp2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135717
Anti-SARS-CoV-2 (COVID-19) nsp2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135717

Cat. No. GTX135717

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) nsp2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909866

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp2 antibody used in Western Blot (WB). GTX135717

GTX135717 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp2 antibody (GTX135717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135717

GTX135717 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp2 antibody detects SARS-CoV-2 (COVID-19) nsp2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp2 stained by SARS-CoV-2 (COVID-19) nsp2 antibody (GTX135717) diluted at 1:2000.

Anti-SARS-CoV-2 (COVID-19) nsp2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135717

GTX135717 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp2 antibody detects SARS-CoV-2 (COVID-19) nsp2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp2 stained by SARS-CoV-2 (COVID-19) nsp2 antibody (GTX135717) diluted at 1:2000.

REFERENCE

Application Reference

Kipfer ET et al. Elife 2023; 12 Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform.
Kipfer E et al. bioRxiv 2023; Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform.
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REVIEW

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Package List Price ($)
$ 399
$ 169