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SARS-CoV-2 (COVID-19) nsp3 antibody

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in IHC (Paraffin sections) (IHC-P). GTX135589
Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135589
Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135589
Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135589

Cat No. GTX135589

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Positive Control

DDDDK-tag tagged SARS-CoV-2 (COVID-19) PLpro (nsp3, partial)-transfected 293T

Product Note

This antibody was raised against nsp3c domain, and it does not react with PLpro domain.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) PLpro (nsp3) (Strain:Wuhan-Hu-1). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2887501

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

COVID-19 PLpro , SARS-coV-2 PLpro , 2019-nCoV PLpro ,2019-nCoV papain-like proteinase , 2019-nCoV nsp3 ,COVID-19 papain-like proteinase , COVID-19 PL-pro , COVID-19 nsp3 , SARS-CoV-2 papain-like proteinase , SARS-CoV-2 PL-pro , SARS-CoV-2 nsp3

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in IHC (Paraffin sections) (IHC-P). GTX135589

GTX135589 IHC-P Image

SARS-CoV-2 (COVID-19) nsp3 antibody detects SARS-CoV-2 (COVID-19) nsp3 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) nsp3 transfected 293T cell FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nsp3 stained by SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135589

GTX135589 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) PLpro (nsp3) antibody (GTX135589) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135589

GTX135589 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nsp3 antibody detects SARS-CoV-2 (COVID-19) nsp3 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) nsp3 transfected 293T cell FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nsp3 stained by SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135589

GTX135589 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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