APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Positive Control
DDDDK-tag tagged SARS-CoV-2 (COVID-19) PLpro (nsp3, partial)-transfected 293T
Product Note
This antibody was raised against nsp3c domain, and it does not react with PLpro domain.
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) PLpro (nsp3) (Strain:Wuhan-Hu-1). The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2887501
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
COVID-19 PLpro , SARS-coV-2 PLpro , 2019-nCoV PLpro ,2019-nCoV papain-like proteinase , 2019-nCoV nsp3 ,COVID-19 papain-like proteinase , COVID-19 PL-pro , COVID-19 nsp3 , SARS-CoV-2 papain-like proteinase , SARS-CoV-2 PL-pro , SARS-CoV-2 nsp3
Research Area
DATA IMAGES
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GTX135589 IHC-P Image
SARS-CoV-2 (COVID-19) nsp3 antibody detects SARS-CoV-2 (COVID-19) nsp3 protein at cytoplasm by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) nsp3 transfected 293T cell FFPE Cell Pellet Block.Green: SARS-CoV-2 (COVID-19) nsp3 stained by SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135589 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) PLpro (nsp3) antibody (GTX135589) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX135589 IHC-P (cell pellet) Image
SARS-CoV-2 (COVID-19) nsp3 antibody detects SARS-CoV-2 (COVID-19) nsp3 protein at cytoplasm by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) nsp3 transfected 293T cell FFPE Cell Pellet Block.Green: SARS-CoV-2 (COVID-19) nsp3 stained by SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135589 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135589). Be the first to share your experience with this product.
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