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SARS-CoV-2 (COVID-19) nsp3 antibody

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135614
Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135614

Cat. No. GTX135614

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against nsp3c domain, and it does not react with PLpro domain.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp3 (Strain:Wuhan-Hu-1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887505

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

COVID-19 PLpro , SARS-coV-2 PLpro , 2019-nCoV PLpro ,2019-nCoV papain-like proteinase , 2019-nCoV nsp3 ,COVID-19 papain-like proteinase , COVID-19 PL-pro , COVID-19 nsp3 , SARS-CoV-2 papain-like proteinase , SARS-CoV-2 PL-pro , SARS-CoV-2 nsp3

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135614

GTX135614 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135614) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135614

GTX135614 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp3 antibody detects SARS-CoV-2 (COVID-19) nsp3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp3 stained by SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135614) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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Package List Price ($)
$ 399
$ 169