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SARS-CoV-2 (COVID-19) nsp3 antibody

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135614
Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135614

Cat. No. GTX135614

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against nsp3c domain, and it does not react with PLpro domain.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp3 (Strain:Wuhan-Hu-1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887505

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

COVID-19 PLpro , SARS-coV-2 PLpro , 2019-nCoV PLpro ,2019-nCoV papain-like proteinase , 2019-nCoV nsp3 ,COVID-19 papain-like proteinase , COVID-19 PL-pro , COVID-19 nsp3 , SARS-CoV-2 papain-like proteinase , SARS-CoV-2 PL-pro , SARS-CoV-2 nsp3

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Western Blot (WB). GTX135614

GTX135614 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135614) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135614

GTX135614 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp3 antibody detects SARS-CoV-2 (COVID-19) nsp3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp3 stained by SARS-CoV-2 (COVID-19) nsp3 antibody (GTX135614) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

N Schmidt et al. Cell 2023; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Application : ICC/IF
Yao Y et al. Antiviral Res 2023; 209 : 105478 RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5'-untranslated region.
Pahmeier F et al. J Virol 2023; 97 (11) : e0087823 Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis.
Manuel Hayn et al. Cell Rep 2021; 35 (7) : 109126 Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities.
Application : WB
Maximilian Hirschenberger et al. STAR Protoc 2021; 2 (4) : 100781 Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins.
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Package List Price ($)
$ 399
$ 169