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SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] used in Western Blot (WB). GTX636839
Anti-SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636839
Anti-SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636839

Cat. No. GTX636839

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1399

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp9. (Strain:Wuhan-hu-1)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910085

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] used in Western Blot (WB). GTX636839

GTX636839 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] (GTX636839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636839

GTX636839 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] detects SARS-CoV-2 (COVID-19) nsp9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 nsp9-transfected 293T.
Green: SARS-CoV-2 (COVID-19) nsp9 stained by SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] (GTX636839) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636839

GTX636839 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] detects SARS-CoV-2 (COVID-19) nsp9 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp9 stained by SARS-CoV-2 (COVID-19) nsp9 antibody [HL1399] (GTX636839) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

H Zhu et al. BMC Biol 2024; 22 (1) : 12 The temporal association of CapZ with early endosomes regulates endosomal trafficking and viral entry into host cells
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations