APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:1000-1:10000 |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture: GTX6353808, Detection: GTX635689
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Not tested in other applications.
Positive Control
DDDDK-tag tagged 2019-nCoV Nucleocapsid-transfected 293T
Product Note
This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
SARS Coronavirus 2
Immunogen
Full length SARS-CoV-2 (COVID-19) nucleocapsid protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888581
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP
Research Area
DATA IMAGES
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GTX635808 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635808 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635808 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.Sensitivity : 39.06 pg/mL
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GTX635808 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) (8*103-9.5*10-3 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 82.34 nM
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REFERENCE
There are currently no references for SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808). Be the first to share your experience with this product.