APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX6353808, Detection: GTX635689
|
Not tested in other applications.
Positive Control
DDDDK-tag tagged 2019-nCoV Nucleocapsid-transfected 293T
Product Note
This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
SARS Coronavirus 2
Immunogen
Full length SARS-CoV-2 (COVID-19) nucleocapsid protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888581
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP
Research Area
DATA IMAGES
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808](/upload/website/prouct_img/middle/GTX635808/GTX635808_44160_20210416_ICC_IF_B_SARSCoronavirus2_21042201_856.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808")
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GTX635808 ICC/IF Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] detects SARS-CoV-2 (COVID-19) nucleocapsid protein at cytoplasm by immunofluorescent analysis.Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:2000.
|
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808](/upload/website/prouct_img/middle/GTX635808/GTX635808_44090_20210416_ICC_IF_B_SARSCoronavirus2_21042201_194.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808")
|
GTX635808 ICC/IF Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] detects SARS-CoV-2 (COVID-19) nucleocapsid protein at cytoplasm by immunofluorescent analysis.Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:2000.
|
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808](/upload/website/prouct_img/middle/GTX635808/GTX635808_44090_20200925_WB_multiplevirus_B_20092903_744.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808")
|
GTX635808 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808](/upload/website/prouct_img/middle/GTX635808/GTX635808_44090_20200925_WB_SARScoronavirus2_B_20092903_649.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808")
|
GTX635808 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808](/upload/website/prouct_img/middle/GTX635808/GTX635808_44090_20201022_ELISA_PAIR_20110422_847.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808")
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GTX635808 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.Sensitivity : 39.06 pg/mL
|
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808](/upload/website/prouct_img/middle/GTX635808/GTX635808_44090_20201022_ELISA_Indirect_EC50_20110422_259.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808")
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GTX635808 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) (8*103-9.5*10-3 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 82.34 nM
|
REFERENCE
There are currently no references for SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808). Be the first to share your experience with this product.
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808](/upload/website/prouct_img/small/GTX635808/GTX635808_44160_20210416_ICC_IF_B_SARSCoronavirus2_21042201_856.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808](/upload/website/prouct_img/small/GTX635808/GTX635808_44090_20210416_ICC_IF_B_SARSCoronavirus2_21042201_194.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808](/upload/website/prouct_img/small/GTX635808/GTX635808_44090_20200925_WB_multiplevirus_B_20092903_744.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808](/upload/website/prouct_img/small/GTX635808/GTX635808_44090_20200925_WB_SARScoronavirus2_B_20092903_649.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808](/upload/website/prouct_img/small/GTX635808/GTX635808_44090_20201022_ELISA_PAIR_20110422_847.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808](/upload/website/prouct_img/small/GTX635808/GTX635808_44090_20201022_ELISA_Indirect_EC50_20110422_259.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808](/upload/website/prouct_img/normal/GTX635808/GTX635808_44160_20210416_ICC_IF_B_SARSCoronavirus2_21042201_856.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808](/upload/website/prouct_img/normal/GTX635808/GTX635808_44090_20210416_ICC_IF_B_SARSCoronavirus2_21042201_194.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808](/upload/website/prouct_img/normal/GTX635808/GTX635808_44090_20200925_WB_multiplevirus_B_20092903_744.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808](/upload/website/prouct_img/normal/GTX635808/GTX635808_44090_20200925_WB_SARScoronavirus2_B_20092903_649.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808](/upload/website/prouct_img/normal/GTX635808/GTX635808_44090_20201022_ELISA_PAIR_20110422_847.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808](/upload/website/prouct_img/normal/GTX635808/GTX635808_44090_20201022_ELISA_Indirect_EC50_20110422_259.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808")
![Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689](/upload/website/prouct_img/small/GTX635689/GTX635689_43978_20200610_ELISA_PAIR_20061602_553.jpg "Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689")
![Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672](/upload/website/prouct_img/small/GTX635672/GTX635672_44067_20210917_ELISA_PAIR_Multiple_21092401_789.jpg "Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] used in ELISA (ELISA). GTX635672")
