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SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113]

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808

Cat No. GTX635808

Host

Mouse

Clonality

Monoclonal

Clone Name

GT113

Isotype

IgG2b

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX6353808, Detection: GTX635689

Not tested in other applications.

Positive Control

DDDDK-tag tagged 2019-nCoV Nucleocapsid-transfected 293T

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888581

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808

GTX635808 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] detects SARS-CoV-2 (COVID-19) nucleocapsid protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:2000.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635808

GTX635808 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] detects SARS-CoV-2 (COVID-19) nucleocapsid protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:2000.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808

GTX635808 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in Western Blot (WB). GTX635808

GTX635808 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808

GTX635808 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] used in ELISA (ELISA). GTX635808

GTX635808 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) (8*103-9.5*10-3 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 82.34 nM

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808). Be the first to share your experience with this product.