*Optimal dilutions/concentrations should be determined by the researcher.
Application
Dilution
WB
1:5000-1:20000
ICC/IF
1:100-1:1000
IHC-P
Assay dependent
IHC-Fr
Assay dependent
ELISA
Assay dependent
Sandwich ELISA
Assay dependent
IHC-P (cell pellet)
Assay dependent
Note :
Sandwich ELISA
Capture : GTX635679, Detection: GTX635678/GTX635688. Please notice that GTX635688/GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.
Product Note
This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
SARS Coronavirus 2
Immunogen
Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Purification
Affinity purified by protein A.
Conjugation
Unconjugated
RRID
AB_2888553
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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GTX635679 ICC/IF Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635679 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 0.074 nM
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GTX635679 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635679 IHC-P Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.The IHC-P was performed by HISTOWIZ.
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GTX635679 IHC-P Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).The IHC-P was performed by HISTOWIZ.
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GTX635679 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
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GTX635679 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL. Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.
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GTX635679 IHC-P (cell pellet) Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635679 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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