SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344]

Name: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344]
Brand: GeneTex
SKU: GTX635679
Price: 369.00 USD

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635679

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635679

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679

Cat No. GTX635679

Reference 6

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL344
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($369), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:500-1:1000
IHC-Fr	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes. Sandwich ELISA Capture : GTX635679, Detection: GTX635678 / GTX635688 / GTX635686-01. Please notice that GTX635688/GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888553

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

GTX635679 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635679 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.074 nM

GTX635679 WB Image

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) as detection antibody at concentration of 1 μg/mL.

GTX635679 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

GTX635679 IHC-P Image

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

REFERENCE

Application Reference

Shapira T. et al. bioRxiv 2021; (Epub) A novel highly potent inhibitor of TMPRSS2-like proteases blocks SARS-CoV-2 variants of concern and is broadly protective against infection and mortality in mice.

Application : ICC/IF

Ruetalo N et al. mSphere 2021; 6 (1) Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients.

Application : WB

Eric Song et al. J Exp Med 2021; 218 (3) : e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.

Application : IHC-Fr

Heaton BE et al. bioRxiv 2020; (Epub) SRSF protein kinases 1 and 2 are essential host factors for human coronaviruses including SARS-CoV-2.

Application : WB

Anand Ramani et al. EMBO J. 2020; : e106230 SARS-CoV-2 targets neurons of 3D human brain organoids.

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REVIEW

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Application Reference

Application : ICC/IF

Ruetalo N et al. mSphere 2021; 6 (1) Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients.

Application : WB

Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.

Application : IHC-Fr

Heaton BE et al. bioRxiv 2020; (Epub) SRSF protein kinases 1 and 2 are essential host factors for human coronaviruses including SARS-CoV-2.

Application : WB

Anand Ramani et al. EMBO J. 2020;:e106230 SARS-CoV-2 targets neurons of 3D human brain organoids.

Eric Song et al. bioRxiv 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.

Application : IHC-Fr

SDS
PBS.pdf

Package	List Price ($)
100 μl	$ 369
25 μl	$ 169
Qty

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344]

Cat No. GTX635679

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX635679 ELISA Image

GTX635679 IHC-P (cell pellet) Image

GTX635679 WB Image

GTX635679 ICC/IF Image

GTX635679 ELISA Image

GTX635679 WB Image

GTX635679 ELISA Image

GTX635679 ELISA Image

GTX635679 IHC-P Image

GTX635679 IHC-P Image

GTX635679 ELISA Image

GTX635679 ELISA Image

REFERENCE

Application Reference

REVIEW

SARS-CoV-2 (COVID-19) nucleocapsid Protein Sandwich ELISA Kit

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511]

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

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