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SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344]

Recombinant antibody Orthogonal
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

Cat. No. GTX635679

Reference 43
2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL344

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:500-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes.

Sandwich ELISA
Capture : GTX635679, Detection : GTX635678 / GTX635688 / GTX635686-01.
Please notice that GTX635688 / GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888553

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635679

GTX635679 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635679

GTX635679 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

GTX635679 WB Image

Orthogonal

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

GTX635679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.074 nM

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

GTX635679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679

GTX635679 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679

GTX635679 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

Application Reference

S Andreu et al. Front Microbiol 2023; 14 : 1185504 Dextran sulfate from Leuconostoc mesenteroides B512F exerts potent antiviral activity against SARS-CoV-2 in vitro and in vivo
Application : ICC/IF
Cloherty APM et al. Emerg Microbes Infect 2023; 12 (1) : 2195020 Berbamine suppresses intestinal SARS-CoV-2 infection via a BNIP3-dependent autophagy blockade.
Application : ICC/IF
Gon?alves R et al. iScience 2023; 26 (2) : 105972 SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of mice.
Sasaki M et al. Sci Transl Med 2023; 15 (679) : eabq4064 S-217622, a SARS-CoV-2 main protease inhibitor, decreases viral load and ameliorates COVID-19 severity in hamsters.
P?rez-Vargas J et al. Antiviral Res 2023; 209 : 105484 Discovery of lead natural products for developing pan-SARS-CoV-2 therapeutics.
Application : ICC/IF
Show More +
Submit a Reference

REVIEW

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] Cat. No. GTX635679

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35264719
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35264719
Submit a Review
Application Reference
S Andreu et al. Front Microbiol 2023; 14:1185504 Dextran sulfate from Leuconostoc mesenteroides B512F exerts potent antiviral activity against SARS-CoV-2 in vitro and in vivo
Application : ICC/IF
Cloherty APM et al. Emerg Microbes Infect 2023; 12 (1):2195020 Berbamine suppresses intestinal SARS-CoV-2 infection via a BNIP3-dependent autophagy blockade.
Application : ICC/IF
Li Z et al. Signal Transduct Target Ther 2023; 8 (1):53 The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation.
Gon?alves R et al. iScience 2023; 26 (2):105972 SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of mice.
Sasaki M et al. Sci Transl Med 2023; 15 (679):eabq4064 S-217622, a SARS-CoV-2 main protease inhibitor, decreases viral load and ameliorates COVID-19 severity in hamsters.
P?rez-Vargas J et al. Antiviral Res 2023; 209:105484 Discovery of lead natural products for developing pan-SARS-CoV-2 therapeutics.
Application : ICC/IF
Garcia G Jr et al. PLoS Biol 2022; 20 (11):e3001851 Hippo signaling pathway activation during SARS-CoV-2 infection contributes to host antiviral response.
Shapira T et al. Mol Biomed 2022; 3 (1):43 Inhibition of glycogen synthase kinase-3-beta (GSK3β) blocks nucleocapsid phosphorylation and SARS-CoV-2 replication.
Application : ICC/IF
Yaron TM et al. Sci Signal 2022; 15 (757):eabm0808 Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.
Application : WB
Shimizu H et al. iScience 2022; 25 (11):105314 LIGHTHOUSE illuminates therapeutics for a variety of diseases including COVID-19.
Application : ICC/IF
Yamamoto Y et al. Int J Mol Sci 2022; 24 (1) Direct Inhibition of SARS-CoV-2 Spike Protein by Peracetic Acid.
Yamamoto Y et al. Int J Mol Sci 2022; 23 (24) SARS-CoV-2 Spike Protein Mutation at Cysteine-488 Impairs Its Golgi Localization and Intracellular S1/S2 Processing.
Chan JF et al. Cell Rep Med 2022; 3 (10):100774 A molecularly engineered, broad-spectrum anti-coronavirus lectin inhibits SARS-CoV-2 and MERS-CoV infection in?vivo.
Ma H et al. Cell Res 2022; 32 (9):831-842 Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including Omicron.
Tirosh Shapira et al. Nature 2022; 605 (7909):340-348 A? TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic.
Lissenya B Argueta et al. iScience 2022; 25 (5):104223 Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy.
Naoya Kitamura et al. J Med Chem 2022; 65 (4):2848-2865 Expedited Approach toward the Rational Design of Noncovalent SARS-CoV-2 Main Protease Inhibitors.
Application : ICC/IF
Ravindra Gupta et al. Res Sq 2022; SARS-CoV-2 Omicron spike mediated immune escape and tropism shift.
Application : WB
Yasui F et al. Sci Rep 2022; 12 (1):4150 Infection with the SARS-CoV-2 B.1.351 variant is lethal in aged BALB/c mice.
Application : IHC-P
Meng B et al. Nature 2022; (Epub) Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts tropism and fusogenicity.
Application : WB
Suzuki R et al. Nature 2022; (Epub) Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant.
Application : WB
Wright T et al. ACS Appl Mater Interfaces 2022; 14 (1):49-56 Photodynamic and Contact Killing Polymeric Fabric Coating for Bacteria and SARS-CoV-2.
Huan Ma et al. J Virol 2021; Potent Neutralization of SARS-CoV-2 by Hetero-bivalent Alpaca Nanobodies Targeting the Spike Receptor-Binding Domain.
Application : ICC/IF
Lissenya B Argueta et al. bioRxiv 2021; SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta.
Application : ICC/IF, IHC-P
Chunlong Ma et al. ACS Cent Sci 2021; 7 (7):1245-1260 Discovery of SARS-CoV-2 Papain-like Protease Inhibitors through a Combination of High-Throughput Screening and a FlipGFP-Based Reporter Assay.
Application : ICC/IF
Mona Seifert et al. Elife 2021; 10 Inhibition of SARS-CoV-2 polymerase by nucleotide analogs from a single-molecule perspective.
Application : ICC/IF
Jiang W et al. Cell Rep 2021; 37 (11):110112 A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge.
Application : IHC-P
Conforti A et al. Mol Ther 2021; (Epub) COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models.
Application : IHC-P
Ohno M et al. Viruses 2021; 13 (11) Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2.
Application : IHC-P
Garc?a-Dorival I et al. Antiviral Res 2021; 194:105167 Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target.
Application : ICC/IF
Asaka MN et al. JCI Insight 2021; 6 (19) Highly susceptible SARS-CoV-2 model in CAG promoter-driven hACE2-transgenic mice.
Application : IHC-P
Tsuji K et al. J Toxicol Sci 2021; 46 (9):425-435 Development of alveolar and airway cells from human iPS cells: toward SARS-CoV-2 research and drug toxicity testing.
Application : ICC/IF
Sasaki M. et al. mBio. 2021;:e0141521 SARS-CoV-2 Bearing a Mutation at the S1/S2 Cleavage Site Exhibits Attenuated Virulence and Confers Protective Immunity.
Application : IHC-P
Sci Immunol 2021; 6 (59) Pharmacological activation of STING blocks SARS-CoV-2 infection.
Application : ICC/IF
Shapira T. et al. bioRxiv 2021; (Epub) A novel highly potent inhibitor of TMPRSS2-like proteases blocks SARS-CoV-2 variants of concern and is broadly protective against infection and mortality in mice.
Application : ICC/IF
Ruetalo N et al. mSphere 2021; 6 (1) Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients.
Application : WB
Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Application : IHC-Fr
Sacco MD et al. Sci Adv 2020; 6 (50) Structure and inhibition of the SARS-CoV-2 main protease reveal strategy for developing dual inhibitors against Mpro and cathepsin L.
Song E et al. bioRxiv 2020; (Epub) Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Sacco MD et al. bioRxiv 2020; Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against Mpro and cathepsin L.
Heaton BE et al. bioRxiv 2020; (Epub) SRSF protein kinases 1 and 2 are essential host factors for human coronaviruses including SARS-CoV-2.
Application : WB
Anand Ramani et al. EMBO J. 2020;:e106230 SARS-CoV-2 targets neurons of 3D human brain organoids.
Eric Song et al. bioRxiv 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.
Application : IHC-Fr
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Package List Price ($)
$ 399
$ 169
Custom Conjugations