SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344]

Name: SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344]
Brand: GeneTex
SKU: GTX635679
Price: 399.00 USD

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635679

Cat. No. GTX635679

Reference 49

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL344
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) nucleocapsid products

View product citations for antibody GTX635679 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:500-1:1000
IHC-Fr	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes. Sandwich ELISA Capture : GTX635679, Detection : GTX635678 / GTX635688 / GTX635686-01. Please notice that GTX635688 / GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

GTX635679 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635679 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

GTX635679 IHC-P Image

GTX635679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.074 nM

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635679 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

GTX635679 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 WB Image

GTX635679 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35264719

GTX635679 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35264719

REFERENCE

Application Reference

Tsumita T et al. Aging Cell 2024; 23 (2) : e14050 Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.

Imbiakha B et al. Sci Adv 2024; 10 (1) : eadg5461 Adaptive immune cells are necessary for SARS-CoV-2-induced pathology.

L Zhang et al. mBio 2024; : e0307123 CRL4B E3 ligase recruited by PRPF19 inhibits SARSCoV-2 infection by targeting ORF6 for ubiquitin-dependent degradation

Application : WB, IHC-P

V Castro et al. ACS Nano 2023; 17 (22) : 22708-22721 Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

Application : ICC/IF

Uemura K et al. Proc Natl Acad Sci U S A 2023; 120 (42) : e2304139120 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses.

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REVIEW

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Application Reference

Tsumita T et al. Aging Cell 2024; 23 (2):e14050 Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.

Imbiakha B et al. Sci Adv 2024; 10 (1):eadg5461 Adaptive immune cells are necessary for SARS-CoV-2-induced pathology.

L Zhang et al. mBio 2024;:e0307123 CRL4B E3 ligase recruited by PRPF19 inhibits SARSCoV-2 infection by targeting ORF6 for ubiquitin-dependent degradation

Application : WB, IHC-P

V Castro et al. ACS Nano 2023; 17 (22):22708-22721 Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

Application : ICC/IF

Uemura K et al. Proc Natl Acad Sci U S A 2023; 120 (42):e2304139120 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses.

Imbiakha B et al. Sci Adv 2023; 9 (38):eadj1736 Age-dependent acquisition of pathogenicity by SARS-CoV-2 Omicron BA.5.

P?rez-Vargas J et al. Emerg Microbes Infect 2023; 12 (2):2246594 A novel class of broad-spectrum active-site-directed 3C-like protease inhibitors with nanomolar antiviral activity against highly immune-evasive SARS-CoV-2 Omicron subvariants.

S Andreu et al. Front Microbiol 2023; 14:1185504 Dextran sulfate from Leuconostoc mesenteroides B512F exerts potent antiviral activity against SARS-CoV-2 in vitro and in vivo