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SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP)

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in Western Blot (WB). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in Western Blot (WB). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

Cat. No. GTX635686-01

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL448

Isotype

IgG

Application

WB, ELISA, Sandwich ELISA

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX635685 / GTX635689 / GTX635679, Detection: GTX635686-01

Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in Western Blot (WB). GTX635686-01

GTX635686-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) diluted at 1:5000.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686-01) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in Western Blot (WB). GTX635686-01

GTX635686-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) diluted at 1:5000.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP) used in ELISA (ELISA). GTX635686-01

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135357-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

REFERENCE

REVIEW

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Package List Price ($)
$ 399