SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP)

Cat. No. GTX635686-01

Reference 7

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL448
Isotype	IgG
Application	WB, ELISA, Sandwich ELISA
Reactivity	SARS Coronavirus, SARS Coronavirus 2
Package	50 μl ($399)

See all SARS-CoV-2 (COVID-19) nucleocapsid products

Datasheet
Reference

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX635685 / GTX635689 / GTX635679, Detection: GTX635686-01

Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888558

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635686-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) diluted at 1:5000.

GTX635686-01 WB Image

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) as detection antibody at concentration of 1 μg/mL.

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135357-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686-01) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

GTX635686-01 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

REFERENCE

Clone Reference

Zhu C et al. Nat Commun 2022; 13 (1):4503 An intranasal ASO therapeutic targeting SARS-CoV-2.

Bullock HA et al. Front Med (Lausanne) 2022; 9:1099408 Probable vertical transmission of Alpha variant of concern (B.1.1.7) with evidence of SARS-CoV-2 infection in the syncytiotrophoblast, a case report.

Rajendra Karki et al. Sci Immunol 2022;:eabo6294 ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection.

Mostafa M Eltobgy et al. Proc Natl Acad Sci U S A 2022; 119 (21):e2202012119 Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and immunothrombosis.

Sarah Reagan-Steiner et al. Emerg Infect Dis 2022; 28 (3):510-517 Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta.

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REVIEW

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Application Reference

Li J et al. ACS Sens 2021; 6 (3):1270-1278 Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.

Clone Reference

Zhu C et al. Nat Commun 2022; 13 (1):4503 An intranasal ASO therapeutic targeting SARS-CoV-2.

Rajendra Karki et al. Sci Immunol 2022;:eabo6294 ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection.

Mostafa M Eltobgy et al. Proc Natl Acad Sci U S A 2022; 119 (21):e2202012119 Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and immunothrombosis.

Sarah Reagan-Steiner et al. Emerg Infect Dis 2022; 28 (3):510-517 Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta.

Vazquez C et al. PLoS One 2021; 16 (6):e0253089 SARS-CoV-2 viral proteins NSP1 and NSP13 inhibit interferon activation through distinct mechanisms.

Package	List Price ($)
50 μl	$ 399
Qty

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] (HRP)

Cat. No. GTX635686-01

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX635686-01 ELISA Image

GTX635686-01 WB Image

GTX635686-01 WB Image

GTX635686-01 ELISA Image

GTX635686-01 ELISA Image

GTX635686-01 ELISA Image

GTX635686-01 ELISA Image

GTX635686-01 ELISA Image

GTX635686-01 ELISA Image

REFERENCE

Clone Reference

REVIEW

Product

Research

Support

Company

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