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SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453]

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687

Cat. No. GTX635687

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL453

Isotype

IgG

Application

WB, ICC/IF, ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2  nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687

GTX635687 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (15.63-1000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635687

GTX635687 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635687

GTX635687 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687

GTX635687 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687

GTX635687 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.006 nM

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687

GTX635687 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687

GTX635687 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687

GTX635687 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] (GTX635687). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] (GTX635687). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations