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SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS]

Recombinant antibody Orthogonal
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

Cat. No. GTX635712

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HL455-MS

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX635685, Detection: GTX635712
Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888569

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635712

GTX635712 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635712

GTX635712 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712

GTX635712 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 (GTX535665), MERS-CoV and SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Orthogonal

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712

GTX635712 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wei Liu et al. J Virol 2021; 95 (12) Activation of STING Signaling Pathway Effectively Blocks Human Coronavirus Infection.
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations