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SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Western Blot (WB). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Western Blot (WB). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Western Blot (WB). GTX635688

Cat No. GTX635688

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL455

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Lateral Flow
This antibody has been validated by lateral flow assay based on the customer’s feedback.

Sandwich ELISA
Capture : GTX635685/GTX635680/GTX635689, Detection: GTX635688. Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685/GTX635680/GTX635689. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Specificity/Sensitivity

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2  nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688

GTX635688 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635688

GTX635688 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Western Blot (WB). GTX635688

GTX635688 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688

GTX635688 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.006 nM

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635688

GTX635688 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688

GTX635688 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635680. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Western Blot (WB). GTX635688

GTX635688 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688

GTX635688 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in ELISA (ELISA). GTX635688

GTX635688 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) (1000-15.63 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] used in Western Blot (WB). GTX635688

GTX635688 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
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