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SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511]

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Lateral Flow (Lateral Flow). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

Cat. No. GTX635689

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL5511

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Lateral Flow
This antibody has been validated by lateral flow assay based on the customer’s feedback.

Sandwich ELISA
Capture : GTX635689, Detection : GTX635678 / GTX635688 / GTX635808 / GTX635686-01 or Capture : GTX635688, Detection : GTX635689. Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing. Gold conjugation version is available upon request.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)
Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635689

GTX635689 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).

Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635689

GTX635689 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Lateral Flow (Lateral Flow). GTX635689

GTX635689 Lateral Flow Image

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.
Pair for S :
Capture : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688)
Detection : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689)
Pair for A:
Capture : Influenza A virus Nucleoprotein antibody [HL1952] (GTX637789)
Detection : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
Pair for B :
Capture : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Detection : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Samples (40 ng) :
1. Lysis buffer
2. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135357-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)
Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) (21.23-0.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.014 nM

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations