SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511]

Name: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511]
Brand: GeneTex
SKU: GTX635689
Price: 369.00 USD

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635689

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635689

Cat No. GTX635689

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL5511
Isotype	IgG
Application	WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($369), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : Lateral Flow This antibody has been validated by lateral flow assay based on the customer’s feedback. Sandwich ELISA Capture : GTX635689, Detection: GTX635678/GTX635688/GTX635808/GTX635686-01. Please notice that GTX635678/GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888561

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.

GTX635689 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.014 nM

GTX635689 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

GTX635689 WB Image

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (1000-15.63 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

GTX635689 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) as detection antibody at concentration of 1 μg/mL.

REFERENCE

Application Reference

Plescia CB et al. J Biol Chem 2020; (Epub) SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles.

Application : WB

Submit a Reference

REVIEW

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Application Reference

Plescia CB et al. J Biol Chem 2020; (Epub) SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles.

Application : WB

SDS
PBS.pdf

Package	List Price ($)
100 μl	$ 369
25 μl	$ 169
Qty

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511]

Cat No. GTX635689

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX635689 ELISA Image

GTX635689 ELISA Image

GTX635689 ICC/IF Image

GTX635689 WB Image

GTX635689 ELISA Image

GTX635689 WB Image

GTX635689 ELISA Image

GTX635689 WB Image

GTX635689 ELISA Image

GTX635689 IHC-P (cell pellet) Image

GTX635689 ELISA Image

REFERENCE

Application Reference

REVIEW

SARS-CoV-2 (COVID-19) Nucleocapsid Protein Sandwich ELISA Kit

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263]

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