SARS-CoV-2 (COVID-19) nucleocapsid antibody

Name: SARS-CoV-2 (COVID-19) nucleocapsid antibody
Brand: GeneTex
SKU: GTX135357
Price: 359.00 USD
Rating: 5 (1 reviews)

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Flow cytometry (FACS). GTX135357

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135357

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunoprecipitation (IP). GTX135357

Cat No. GTX135357

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus, SARS Coronavirus 2
Package	100 μl ($359), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	Assay dependent
IP	1:100-1:5000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : Sandwich ELISA Capture : GTX632269/GTX135357, Detection: GTX135357/GTX632269

Not tested in other applications.

Specificity/Sensitivity

This antibody detects SARS-CoV-2 nucleocapsid protein. Our internal testing indicates cross-reactivity with SARS-CoV nucleocapsid protein, but not with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

GTX135357 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX135357 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX135357 FACS Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) detects SARS-CoV-2 (COVID-19) nucleocapsid by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) dilution: 1:100.

GTX135357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX135357 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX135357 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX135357 WB Image

GTX135357 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX135357 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX135357 IP Image

Immunoprecipitation of SARS-CoV-2 (COVID-19) nucleocapsid protein from SARS-CoV-2 (2019-nCoV) NP-transfected 293T whole cell extract using 1 μg of SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357).
Western blot analysis was performed using SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

GTX135357 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Thomas Mandel Clausen et al. Cell 2020; S0092-8674 (20) : 31230-7 SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2.

Application : FACS

Robbiani DF et al. bioRxiv 2020; (Epub) Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.

Application : ICC/IF

Kizzmekia S Corbett et al. N Engl J Med . 2020; (Epub) Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates.

Application : IHC-P

Davide F. Robbiani et al. bioRxiv 2020; (Epub) Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.

Application : ICC/IF

Reactivity : SARS Coronavirus 2

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