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SARS-CoV-2 (COVID-19) nucleocapsid antibody

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Flow cytometry (FACS). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunoprecipitation (IP). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135357

Cat No. GTX135357

Reference 44
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 Coronavirus, SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($359),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1:100-1:5000
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632269 / GTX135357, Detection : GTX135357 / GTX632269
Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein. Our internal testing indicates cross-reactivity with SARS-CoV nucleocapsid protein, but not with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2868464

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

GTX135357 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

GTX135357 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Flow cytometry (FACS). GTX135357

GTX135357 FACS Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) detects SARS-CoV-2 (COVID-19) nucleocapsid by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) dilution: 1:100.
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

GTX135357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357

GTX135357 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357

GTX135357 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunoprecipitation (IP). GTX135357

GTX135357 IP Image

Immunoprecipitation of SARS-CoV-2 (COVID-19) nucleocapsid protein from SARS-CoV-2 (2019-nCoV) NP-transfected 293T whole cell extract using 1 μg of SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357).
Western blot analysis was performed using SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

GTX135357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

GTX135357 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

GTX135357 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135357

GTX135357 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Madden PJ et al. bioRxiv 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
Application : IHC-Fr
L?pez-Mu?oz AD et al. bioRxiv 2021; Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity.
Piscotta FJ et al. mSphere 2021; 6 (6) : e0071121 Metabolites with SARS-CoV-2 Inhibitory Activity Identified from Human Microbiome Commensals.
Application : ICC/IF
Wagner TR et al. EMBO Rep 2021; : e53865 Biparatopic nanobodies protect mice from lethal challenge with SARS-CoV-2 variants of concern.
Application : ICC/IF
Yewdell J et al. Res Sq 2021; (Epub) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity.
Show More +
Submit a Reference

REVIEW

SARS-CoV-2 (COVID-19) nucleocapsid antibody Cat No. GTX135357

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC (Paraffin sections) (IHC-P). GTX135357
Date : Anonymous submitted on 07-Mar-2022
Score :
Application Tested : IHC-P
Sample : Huma skin tissue specimen
Blocking : 0.3% H2O2 meta, RT, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate buffer (ph 6.0)
Primary Antibody : 1:500, 4°C, > 12Hr
Lane Description : Eczema after vaccinated. The patient was COVID-19 negative.
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Flow cytometry (FACS). GTX135357
Date : Anonymous submitted on 14-Jul-2020
Score :
Application Tested : FACS
Sample Species : SARS-CoV-2
Sample : Vero E6 cells infected with SARS-CoV-2 USA-WA1/2020
Fixation : 4% formaldehyde in PBS
Permeabilization /Antigen retrieval : BD Cytofix/Cytoperm
Primary Antibody : 1:100, 25°C, 0.5Hr
Submit a Review
Application Reference
Madden PJ et al. bioRxiv 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
Application : IHC-Fr
L?pez-Mu?oz AD et al. bioRxiv 2021; Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity.
Piscotta FJ et al. mSphere 2021; 6 (6):e0071121 Metabolites with SARS-CoV-2 Inhibitory Activity Identified from Human Microbiome Commensals.
Application : ICC/IF
Wagner TR et al. EMBO Rep 2021;:e53865 Biparatopic nanobodies protect mice from lethal challenge with SARS-CoV-2 variants of concern.
Application : ICC/IF
Yewdell J et al. Res Sq 2021; (Epub) Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity.
Gagne M et al. Cell 2021; Protection from SARS-CoV-2 Delta one year after mRNA-1273 vaccination in rhesus macaques coincides with anamnestic antibody response in the lung.
Application : IHC-P
Saiz ML et al. Clin Epigenetics 2021; 13 (1):187 Epigenetic targeting of the ACE2 and NRP1 viral receptors limits SARS-CoV-2 infectivity.
Application : WB
Peng YC et al. Diagnostics (Basel) 2021; 11 (10) A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW).
Application : WB
Kmiec D et al. PLoS Pathog 2021; 17 (10):e1009726 S-farnesylation is essential for antiviral activity of the long ZAP isoform against RNA viruses with diverse replication strategies.
Application : WB
Carapito R et al. Sci Transl Med 2021;:eabj7521 Identification of driver genes for critical forms of COVID-19 in a deeply phenotyped young patient cohort.
Application : ICC/IF
Cantwell AM et al. J Virol 2021; 95 (20):e0101021 Kinetic Multi-omic Analysis of Responses to SARS-CoV-2 Infection in a Model of Severe COVID-19.
Application : WB
Corbett KS et al. Nat Immunol 2021; 22 (10):1306-1315 mRNA-1273 protects against SARS-CoV-2 beta infection in nonhuman primates.
Application : IHC-P
Corbett KS et al. Science 2021; 373 (6561):eabj0299 Immune correlates of protection by mRNA-1273 vaccine against SARS-CoV-2 in nonhuman primates.
Application : IHC-P
Francica JR et al. Sci Transl Med 2021; 13 (607) Protective antibodies elicited by SARS-CoV-2 spike protein vaccination are boosted in the lung after challenge in nonhuman primates.
Application : IHC-P
Junqueira C et al. Res Sq 2021; (Epub) SARS-CoV-2 infects blood monocytes to activate NLRP3 and AIM2 inflammasomes, pyroptosis and cytokine release.
Verma R et al. mSystems 2021; 6 (4):e0064321 RNA-Protein Interaction Analysis of SARS-CoV-2 5' and 3' Untranslated Regions Reveals a Role of Lysosome-Associated Membrane Protein-2a during Viral Infection.
Application : WB
Eriksen A.Z. et al. Cell Stem Cell 2021; 28 (7):1205-1220 SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium.
Application : IHC-P
DiPiazza A.T. et al. Immunity 2021; S1074-7613 (21):00262-4 COVID-19 vaccine mRNA-1273 elicits a protective immune profile in mice that is not associated with vaccine-enhanced disease upon SARS-CoV-2 challenge.
Application : IHC-P
Li D. et al. Cell 2021; S0092-8674 (21):00756-X In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies.
Application : IHC-P
Wang Z et al. Nature 2021; 595 (7876):426-431 Naturally enhanced neutralizing breadth against SARS-CoV-2 one year after infection.
Application : ICC/IF
Sci Immunol 2021; 6 (59) Pharmacological activation of STING blocks SARS-CoV-2 infection.
Sukhikh G et al. Viruses 2021; 13 (3) Vertical Transmission of SARS-CoV-2 in Second Trimester Associated with Severe Neonatal Pathology.
Application : IHC-P
Saunders KO et al. Nature 2021; 594 (7864):553-559 Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses.
Application : IHC-P
Park SH et al. PLoS Pathog 2021; 17 (5):e1009519 Interactions of SARS-CoV-2 envelope protein with amilorides correlate with antiviral activity.
Application : ICC/IF
Mellott DM et al. ACS Chem Biol 2021; 16 (4):642-650 A Clinical-Stage Cysteine Protease Inhibitor blocks SARS-CoV-2 Infection of Human and Monkey Cells.
Hoffmann H. et al. Cell 2021; 184 (1):133-148 TMEM41B Is a Pan-flavivirus Host Factor.
Application : ICC/IF
Rosado-Olivieri E. et al. Cell 2021; (Epub) Self-Organizing, Symmetry Breaking, Isogenic Human Lung Buds on Microchips Identify Alveolar Stem Cells as Novel Targets of SARS-CoV-2.
Li J et al. ACS Sens 2021; 6 (3):1270-1278 Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.
Meyer M et al. bioRxiv 2021; (Epub) mRNA-1273 efficacy in a severe COVID-19 model: attenuated activation of pulmonary immune cells after challenge.
Application : IHC-P
Lu-Culligan A et al. medRxiv 2021; (Epub) SARS-CoV-2 infection in pregnancy is associated with robust inflammatory response at the maternal-fetal interface.
Application : ICC/IF
Sukhikh G et al. Viruses 2021; 13 (3):447 Vertical Transmission of SARS-CoV-2 in Second Trimester Associated with Severe Neonatal Pathology.
Application : IHC-P
Mor M et al. PLoS Pathog 2021; 17 (2):e1009165 Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors.
Application : ICC/IF
Hoffmann HH et al. bioRxiv 2020; (Epub) TMEM41B is a pan-flavivirus host factor.
Mor M et al. bioRxiv 2020; (Epub) Multi-Clonal Live SARS-CoV-2 In Vitro Neutralization by Antibodies Isolated from Severe COVID-19 Convalescent Donors.
Wang Z et al. bioRxiv 2020; (Epub) Enhanced SARS-CoV-2 Neutralization by Secretory IgA in vitro.
Schmidt F et al. J Exp Med 2020; 217 (11) Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.
Schmidt F et al. bioRxiv 2020; Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.
Plescia CB et al. J Biol Chem 2020; (Epub) SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles.
Application : WB, ICC/IF
Reactivity : Coronavirus
William M Schneider et al. Cell 2020; (Epub) Genome-scale identification of SARS-CoV-2 and pan-coronavirus host factor networks.
Application : ICC/IF
Robbiani DF et al. Nature 2020; 584 (7821):437-442 Convergent antibody responses to SARS-CoV-2 in convalescent individuals.
Application : ICC/IF
Thomas Mandel Clausen et al. Cell 2020; S0092-8674 (20):31230-7 SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2.
Application : FACS
Robbiani DF et al. bioRxiv 2020; (Epub) Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.
Application : ICC/IF
Kizzmekia S Corbett et al. N Engl J Med . 2020; (Epub) Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates.
Application : IHC-P
Davide F. Robbiani et al. bioRxiv 2020; (Epub) Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals.
Application : ICC/IF
Reactivity : SARS Coronavirus 2
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 359
$ 169