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SARS-CoV-2 (COVID-19) nucleocapsid antibody

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Flow cytometry (FACS). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunoprecipitation (IP). GTX135357
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

Cat No. GTX135357

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:5000
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632269/GTX135357, Detection: GTX135357/GTX632269

Not tested in other applications.

Specificity/Sensitivity

This antibody detects SARS-CoV-2 nucleocapsid protein. Our internal testing indicates cross-reactivity with SARS-CoV nucleocapsid protein, but not with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

GTX135357 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

GTX135357 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Flow cytometry (FACS). GTX135357

GTX135357 FACS Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) detects SARS-CoV-2 (COVID-19) nucleocapsid by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) dilution: 1:100.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

GTX135357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357

GTX135357 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135357

GTX135357 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

GTX135357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in ELISA (ELISA). GTX135357

GTX135357 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135357

GTX135357 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Immunoprecipitation (IP). GTX135357

GTX135357 IP Image

Immunoprecipitation of SARS-CoV-2 (COVID-19) nucleocapsid protein from SARS-CoV-2 (2019-nCoV) NP-transfected 293T whole cell extract using 1 μg of SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357).
Western blot analysis was performed using SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody used in Western Blot (WB). GTX135357

GTX135357 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SARS-CoV-2 (COVID-19) nucleocapsid antibody Cat No. GTX135357

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Jul-2020
Score :
Application Tested : FACS
Sample Species : SARS-CoV-2
Sample : Vero E6 cells infected with SARS-CoV-2 USA-WA1/2020
Fixation : 4% formaldehyde in PBS
Permeabilization /Antigen retrieval : BD Cytofix/Cytoperm
Primary Antibody : 1:100, 25°C, 0.5Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf