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SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro
SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

Cat No. GTX135592-pro

Application

ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX635685, Detection: GTX635712/GTX635688/GTX635678.
Please notice that GTX635688/GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal His tagged full length protein of YP_009724397.2

Expression System

HEK293 cells

Purity

>90%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro Image

0.5 μg of GTX135592-pro SARS-CoV-2 (COVID-19) nucleocapsid protein analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Sentivity : 2.68 pg/mL

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro Image

SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag. GTX135592-pro

GTX135592-pro ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL
Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635808. Please contact us for custom HRP-conjugated antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf