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SARS-CoV-2 (COVID-19) Spike antibody

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in ELISA (ELISA). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in ELISA (ELISA). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in ELISA (ELISA). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Western Blot (WB). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Western Blot (WB). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Western Blot (WB). GTX135356
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135356

Cat. No. GTX135356

Reference 28
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604, Detection: GTX135356
Not tested in other applications.

Positive Control

SARS-CoV-2 (2019-nCoV) Spike-transfected 293T

Product Note

This antibody detects SARS-CoV-2 spike protein (S1 subunit), but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887482

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in ELISA (ELISA). GTX135356

GTX135356 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) spike protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in ELISA (ELISA). GTX135356

GTX135356 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in ELISA (ELISA). GTX135356

GTX135356 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135356 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135356

GTX135356 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) spike antibody detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Western Blot (WB). GTX135356

GTX135356 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Western Blot (WB). GTX135356

GTX135356 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Western Blot (WB). GTX135356

GTX135356 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135356

GTX135356 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Cheedarla N et al. iScience 2023; 26 (11) : 108256 Rapid, high throughput, automated detection of SARS-CoV-2 neutralizing antibodies against Wuhan-WT, delta and omicron BA1, BA2 spike trimers.
SB Park et al. Commun Biol 2023; 6 (1) : 556 SARS-CoV-2 omicron variants harbor spike protein mutations responsible for their attenuated fusogenic phenotype
Arai Y et al. iScience 2023; 26 (1) : 105742 Stimulation of interferon-β responses by aberrant SARS-CoV-2 small viral RNAs acting as retinoic acid-inducible gene-I agonists.
Merino VF et al. Antiviral Res 2023; 211 : 105550 Nucleolin mediates SARS-CoV-2 replication and viral-induced apoptosis of host cells.
Application : IHC-P
Gellenoncourt S et al. J Virol 2022; 96 (19) : e0130122 The Spike-Stabilizing D614G Mutation Interacts with S1/S2 Cleavage Site Mutations To Promote the Infectious Potential of SARS-CoV-2 Variants.
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Submit a Reference

REVIEW

SARS-CoV-2 (COVID-19) Spike antibody Cat. No. GTX135356

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-SARS-CoV-2 (COVID-19) Spike antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135356
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : ICC/IF
Sample Species : SARS-CoV-2
Sample : SARS-CoV-2 FL spike transfected HeLa cells
Primary Antibody : 1μg/ml, 4°C, 16Hr
Submit a Review
Application Reference
Cheedarla N et al. iScience 2023; 26 (11):108256 Rapid, high throughput, automated detection of SARS-CoV-2 neutralizing antibodies against Wuhan-WT, delta and omicron BA1, BA2 spike trimers.
SB Park et al. Commun Biol 2023; 6 (1):556 SARS-CoV-2 omicron variants harbor spike protein mutations responsible for their attenuated fusogenic phenotype
Arai Y et al. iScience 2023; 26 (1):105742 Stimulation of interferon-β responses by aberrant SARS-CoV-2 small viral RNAs acting as retinoic acid-inducible gene-I agonists.
Merino VF et al. Antiviral Res 2023; 211:105550 Nucleolin mediates SARS-CoV-2 replication and viral-induced apoptosis of host cells.
Application : IHC-P
Hayashi H et al. Sci Rep 2022; 12 (1):20923 Modified DNA vaccine confers improved humoral immune response and effective virus protection against SARS-CoV-2 delta variant.
Application : WB
Prout A et al. Vaccine 2022; 40 (37):5529-5536 Functional profiling of Covid 19 vaccine candidate by flow virometry.
Application : FACS
Gao SY et al. Front Immunol 2022; 13:1038562 Nanocell COVID-19 vaccine triggers a novel immune response pathway producing high-affinity antibodies which neutralize all variants of concern.
Ruiz MJ et al. Front Immunol 2022; 13:842468 Persistent but dysfunctional mucosal SARS-CoV-2-specific IgA and low lung IL-1β associate with COVID-19 fatal outcome: A cross-sectional analysis.
Kumar CS et al. ACS Infect Dis 2022; 8 (10):2119-2132 Virus-Like Particles of SARS-CoV-2 as Virus Surrogates: Morphology, Immunogenicity, and Internalization in Neuronal Cells.
Vakhrusheva AV et al. Vaccines (Basel) 2022; 10 (8) SARS-CoV-2 Subunit Virus-like Vaccine Demonstrates High Safety Profile and Protective Efficacy: Preclinical Study.
Imanishi I et al. BMC Vet Res 2022; 18 (1):443 Guest edited collection serological study of SARS-CoV-2 antibodies in japanese cats using protein-A/G-based ELISA.
Application : ELISA
Park H et al. Adv Sci (Weinh) 2022; 9 (24):e2105320 TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.
Application : ICC/IF
Ishigaki H et al. Front Microbiol 2022; 13:967019 An attenuated vaccinia vaccine encoding the severe acute respiratory syndrome coronavirus-2 spike protein elicits broad and durable immune responses, and protects cynomolgus macaques and human angiotensin-converting enzyme 2 transgenic mice from severe acute respiratory syndrome coronavirus-2 and its variants.
Gellenoncourt S et al. J Virol 2022; 96 (19):e0130122 The Spike-Stabilizing D614G Mutation Interacts with S1/S2 Cleavage Site Mutations To Promote the Infectious Potential of SARS-CoV-2 Variants.
Weijie Zeng et al. Front Cell Infect Microbiol 2022; 12:911313 Evidence of Infection of Human Embryonic Stem Cells by SARS-CoV-2.
Application : ICC/IF
Viviana V?squez et al. Anal Chim Acta 2022; 1205:339718 SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex.
Ordonez AA et al. Commun Biol 2022; 5 (1):242 Sulforaphane exhibits antiviral activity against pandemic SARS-CoV-2 and seasonal HCoV-OC43 coronaviruses in vitro and in mice.
Application : IHC-P
Yang B et al. Proc Natl Acad Sci U S A 2022; 119 (4) SNX27 suppresses SARS-CoV-2 infection by inhibiting viral lysosome/late endosome entry.
Nanda Kishore Routhu et al. Immunity 2021; A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs.
Application : FACS
Miao-Hsi Hsieh et al. Front Immunol 2021; 12:641360 Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.
Application : WB
Lin WS et al. Front Immunol 2021; 12:795741 Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.
Application : WB
Meseda CA et al. NPJ Vaccines 2021; 6 (1):145 MVA vector expression of SARS-CoV-2 spike protein and protection of adult Syrian hamsters against SARS-CoV-2 challenge.
Huang HC et al. EBioMedicine 2021; 74:103712 Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.
Application : WB
Sci Immunol 2021; 6 (59) Pharmacological activation of STING blocks SARS-CoV-2 infection.
Application : ICC/IF
Sukhikh G et al. Viruses 2021; 13 (3) Vertical Transmission of SARS-CoV-2 in Second Trimester Associated with Severe Neonatal Pathology.
Application : IHC-P
Harbour JC et al. J Immunol 2021; 206 (11):2596-2604 Cellular and Humoral Immune Responses in Mice Immunized with Vaccinia Virus Expressing the SARS-CoV-2 Spike Protein.
Application : WB
Garc?a-Arriaza J et al. J Virol 2021; (Epub) COVID-19 vaccine candidates based on modified vaccinia virus Ankara expressing the SARS-CoV-2 spike induce robust T- and B-cell immune responses and full efficacy in mice.
Application : WB, ICC/IF
Sukhikh G et al. Viruses 2021; 13 (3):447 Vertical Transmission of SARS-CoV-2 in Second Trimester Associated with Severe Neonatal Pathology.
Application : IHC-P
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169